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Mixed mode monolithic sorbent in pipette tip for extraction of ractopamine and clenbuterol prior to analysis by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap MS
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Document Title
Mixed mode monolithic sorbent in pipette tip for extraction of ractopamine and clenbuterol prior to analysis by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap MS
Author
Ar-sanork K., Karuwan C., Surapanich N., Wilairat P., Nacapricha D., Chaisuwan P.
Name from Authors Collection
Affiliations
School of Chemistry, Institute of Science, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima, 30000, Thailand; Graphene and Printed Electronics for Dual-Use Applications Research Division (GPERD), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand; Flow Innovation-Research for Science Technology Laboratories (FIRST Labs.), Bangkok, Thailand; Department of Chemistry, Faculty of Science and Technology, Rajanagarindra Rajabhat University, 22 Maruphong Road,Tambon Na Mueang, Muang, Chachoengsao, 24000, Thailand; Analytical Sciences and National Doping Test Institute, Mahidol University, Rama VI Rd, Bangkok, 10400, Thailand; Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, 10400, Thailand
Type
Article
Source Title
Journal of Analytical Science and Technology
ISSN
20933134
Year
2021
Volume
12
Issue
1
Open Access
Gold
Publisher
Springer Science and Business Media Deutschland GmbH
DOI
10.1186/s40543-021-00275-5
Format
Abstract
This work developed a fast and simple method for the quantification of two important β-agonists, ractopamine (RAC) and clenbuterol (CLEN) in animal urines. The method was based on micro-extraction by a mixed mode monolithic material and analysis by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap mass spectrometer. A mixed functional monolith with nonpolar ester-based structure containing polar carboxylic groups was thermally synthesized in situ in the pipette tips. The material combines both hydrophobic and ionic interactions. Parameters including conditions and composition of reagents for the in-pipette tip monolith synthesis, as well as the extraction process, were investigated and optimized. The procedure for extraction is 800 μL washing solvent of 10:90 (v/v) ACN:water and 150 μL eluting solvent of 30:70 (v/v) ACN:200 mM acetate buffer pH 4.0. Extraction efficiencies of 92% and 100% for RAC and CLEN, respectively, were achieved within 5 min with total organic solvent consumption of 395 μL. The extracts of spiked cattle and swine urines were analyzed by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap mass spectrometer. Good recovery with acceptable precision was observed. The mass spectrometry data confirmed efficient matrix removal by the synthesized extraction sorbent allowing routine analysis by the HPLC-UV method. © 2021, The Author(s).
Keyword
Clenbuterol | HPLC analysis | Micro-solid phase extraction | Monolith | Ractopamine
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Suranaree University of Technology; South Australian Research and Development Institute
License
CC BY
Rights
Author
Publication Source
Scopus
Note
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