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A simple label-free electrochemical sensor for sensitive detection of alpha-fetoprotein based on specific aptamer immobilized platinum nanoparticles/carboxylated-graphene oxide
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Document Title
A simple label-free electrochemical sensor for sensitive detection of alpha-fetoprotein based on specific aptamer immobilized platinum nanoparticles/carboxylated-graphene oxide
Author
Upan J., Youngvises N., Tuantranont A., Karuwan C., Banet P., Aubert P.-H., Jakmunee J.
Name from Authors Collection
Scopus Author ID
8532633300
Affiliations
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand; Thammasat University Research Unit in Carbon Materials and Green Chemistry Innovations, Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathum Thani, 12120, Thailand; Graphene and Printed Electronics for Dual-Use Applications Research Division, National Security and Dual-Use Technology Center, National Science and Technology Development Agency, Pathumthani, 12120, Thailand; Center of Advanced Materials of Printed Electronics and Sensors, Materials Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand; CY Cergy Paris Université, LPPI, Cergy-Pontoise, 95000, France; Center of Excellence for Innovation in Chemistry, and Center of Chemistry for Development of Health Promoting Products From Northern Resources, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand
Type
Article
Source Title
Scientific Reports
ISSN
20452322
Year
2021
Volume
11
Issue
1
Open Access
Gold, Green
Publisher
Nature Research
DOI
10.1038/s41598-021-93399-y
Abstract
A label-free electrochemical aptamer-based sensor has been fabricated for alpha-fetoprotein (AFP) detection. Platinum nanoparticles on carboxylated-graphene oxide (PtNPs/GO-COOH) modified screen-printed graphene-carbon paste electrode (SPGE) was utilized as an immobilization platform, and the AFP aptamer was employed as a bio-recognition element. The synthesized GO-COOH helps to increase the surface area and amounts of the immobilized aptamer. Subsequently, PtNPs are decorated on GO-COOH to enhance electrical conductivity and an oxidation current of the hydroquinone electrochemical probe. The aptamer selectively interacts with AFP, causing a decrease in the peak current of the hydroquinone because the binding biomolecules on the electrode surface hinder the electron transfer of the redox probe. Effects of aptamer concentration and AFP incubation time were studied, and the current changes of the redox probe before and after AFP binding were investigated by square wave voltammetry. The developed aptasensor provides a linear range from 3.0–30 ng mL−1 with a detection limit of 1.22 ng mL−1. Moreover, the aptamer immobilized electrode offers high selectivity to AFP molecules, good stability, and sensitive determination of AFP in human serum samples with high recoveries. © 2021, The Author(s).
Funding Sponsor
Chiang Mai University; National Science and Technology Development Agency; Faculty of Agro-Industry, Chiang Mai University; Faculty of Veterinary Medicine, Chiang Mai University; Faculty of Science, Chiang Mai University; Graduate School, Chiang Mai University; Faculty of Medicine, Chiang Mai University; Center of Excellence for Innovation in Chemistry; Functional Food Research Center for Well-being, Chiang Mai University; Department of Chemistry, Faculty of Science, Chiang Mai University; Materials Science Research Center, Faculty of Science, Chiang Mai University
License
CC BY
Rights
Author
Publication Source
Scopus