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Genetic diversity and molecular markers in introduced and Thai native apple snails (Pomacea and Pila)
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Document Title
Genetic diversity and molecular markers in introduced and Thai native apple snails (Pomacea and Pila)
Author
Thaewnon-Ngiw B., Klinbunga S., Phanwichien K., Sangduen N., Lauhachinda N., Menasveta P.
Name from Authors Collection
Affiliations
Program of Bioscience, Graduate School, Kasetsart University, Bangkok 10900, Thailand; Marine Biotechnology Research Unit, Natl. Ctr. Genetic Eng. and Biotech., Natl. Sci. and Technol. Devmt. Agy., 113 Paholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand; Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand; Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand; Department of General Science, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand; Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
Type
Article
Source Title
Journal of Biochemistry and Molecular Biology
ISSN
12258687
Year
2004
Volume
37
Issue
4
Page
493-502
Open Access
Bronze
Publisher
Springer Verlag
DOI
10.5483/bmbrep.2004.37.4.493
Abstract
The genetic diversity and species-diagnostic markers in the introduced apple snail, Pomacea canaliculata and in the native Thai apple snails; Pila ampullacea, P. angelica, P. pesmei, and P. polita, were investigated by restriction analysis of COI and are reported for the first time. Twenty-one composite haplotypes showing non-overlapping distributions among species were found. Genetic heterogeneity analysis indicated significant differences between species (P < 0.0001) and within P. pesmei (P < 0.0001) and P. angelica (P 0.0036 as modified by the Bonferroni procedure), P. ampullacea (P = 0.0824-1.000) WAA P. polita (P = 1.0000). A neighbor-joining tree based on genetic distance between pairs of composite haplotypes differentiated all species and indicated that P. angelica and P. pesmei are closely related phylogenetically. In addition, the 16S rDNA of these species was cloned and sequenced. A species-specific PCR for P. canaliculata was successfully developed with a sensitivity of detection of approximately 50 pg of the target DNA template. The amplification of genomic DNA (50 pg and 25 ng) isolated from the fertilized eggs, and juveniles (1, 7, and 15 d after hatching) of Pomacea canaliculata was also successful, and suggested that Pomacea canaliculata and Pila species can be discriminated from the early stages of development.
Keyword
Apple snails | Genetic diversity | PCR-RFLP | Pila | Pomacea
License
CC BY-NC
Rights
Author
Publication Source
Scopus