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Phenoloxidase is an important component of the defense against Aeromonas hydrophila infection in a crustacean, Pacifastacus leniusculus
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Document Title
Phenoloxidase is an important component of the defense against Aeromonas hydrophila infection in a crustacean, Pacifastacus leniusculus
Author
Liu H., Jiravanichpaisal P., Cerenius L., Bok L.L., Söderhäll I., Söderhäll K.
Name from Authors Collection
Affiliations
Dept. of Comparative Physiology, Uppsala University, Norbyvägen 18A, SE-752 36 Uppsala, Sweden; Molecular Aquatic Biology and Genetic Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 73/1 Rama VI Road, Rajdhevee, Bangkok 10400, Thailand; National Research Laboratory of Defense Proteins, College of Pharmacy, Pusan National University, Kumjeong Ku, Busan 609-735, South Korea
Type
Article
Source Title
Journal of Biological Chemistry
ISSN
00219258
Year
2007
Volume
282
Issue
46
Page
33593-33598
Open Access
Hybrid Gold
DOI
10.1074/jbc.M706113200
Abstract
The melanization cascade, in which phenoloxidase is the terminal enzyme, appears to play a key role in recognition of and defense against microbial infections in invertebrates. Here, we show that phenoloxidase activity and melanization are important for the immune defense toward a highly pathogenic bacterium, Aeromonas hydrophila, in the freshwater crayfish, Pacifastacus leniusculus. RNA interference-mediated depletion of crayfish prophenoloxidase leads to increased bacterial growth, lower phagocytosis, lower phenoloxidase activity, lower nodule formation, and higher mortality when infected with this bacterium. In contrast, if RNA interference of pacifastin, an inhibitor of the crayfish prophenoloxidase activation cascade, is performed, it results in lower bacterial growth, increased phagocytosis, increased nodule formation, higher phenoloxidase activity, and delayed mortality. Our data therefore suggest that phenoloxidase is required in crayfish defense against an infection by A. hydrophila, a highly virulent and pathogenic bacterium to crayfish. © 2007 by The American Society for Biochemistry and Molecular Biology, Inc.
License
CC BY or CC BY-NC-ND
Rights
Elsevier B.V.
Publication Source
Scopus