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Plasma phosphoproteome and differential plasma phosphoproteins with Opisthorchis viverrini-related cholangiocarcinoma
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Document Title
Plasma phosphoproteome and differential plasma phosphoproteins with Opisthorchis viverrini-related cholangiocarcinoma
Author
Kotawong K., Thitapakorn V., Roytrakul S., Phaonakrop N., Viyanant V., Na-Bangchang K.
Name from Authors Collection
Affiliations
Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Pathumthani, Thailand; Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma, Chulabhorn International College of Medicine, Pathumthani, Thailand; Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani, Thailand; Excellence Center in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma, Thammasat University, Pathumthani, Thailand
Type
Article
Source Title
Asian Pacific Journal of Cancer Prevention
ISSN
15137368
Year
2015
Volume
16
Issue
3
Page
1011-1018
Open Access
Gold, Green
Publisher
Asian Pacific Organization for Cancer Prevention
DOI
10.7314/APJCP.2015.16.3.1011
Abstract
This study was conducted to investigate the plasma phosphoproteome and differential plasma phosphoproteins in cases of of Opisthorchis viverrini (OV)-related cholangiocarcinoma (CCA). Plasma phosphoproteomes from CCA patients (10) and non-CCA subjects (5 each for healthy subjects and OV infection) were investigated using gel-based and solution-based LC-MS/MS. Phosphoproteins in plasma samples were enriched and analyzed by LC-MS/MS. STRAP, PANTHER, iPath, and MeV programs were applied for the identification of their functions, signaling and metabolic pathways; and for the discrimination of potential biomarkers in CCA patients and non-CCA subjects, respectively. A total of 90 and 60 plasma phosphoproteins were identified by gel-based and solution-based LC-MS/MS, respectively. Most of the phosphoproteins were cytosol proteins which play roles in several cellular processes, signaling pathways, and metabolic pathways (STRAP, PANTHER, and iPath analysis). The absence of serine/arginine repetitive matrix protein 3 (A6NNA2), tubulin tyrosine ligase-like family, member 6, and biorientation of chromosomes in cell division protein 1-like (Q8NFC6) in plasma phosphoprotein were identified as potential biomarkers for the differentiation of healthy subjects from patients with CCA and OV infection. To differentiate CCA from OV infection, the absence of both serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform and coiled-coil domain-containing protein 126 precursor (Q96EE4) were then applied. A combination of 5 phosphoproteins may new alternative choices for CCA diagnosis.
Keyword
Biomarkers | Cholangiocarcinoma | LC-MS/MS | Phosphoproteomes
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
West Asia Organization for Cancer Prevention
Publication Source
Scopus