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Anti-malarial effect of 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one and green tea extract on erythrocyte-stage Plasmodium berghei in mice
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Document Title
Anti-malarial effect of 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one and green tea extract on erythrocyte-stage Plasmodium berghei in mice
Author
Thipubon P., Tipsuwan W., Uthaipibull C., Santitherakul S., Srichairatanakool S.
Name from Authors Collection
Affiliations
Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Science Park, Pathumthani, Thailand; Medical Science Research Equipment Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand
Type
Article
Source Title
Asian Pacific Journal of Tropical Biomedicine
ISSN
22211691
Year
2015
Volume
5
Issue
11
Page
932-936
Open Access
Gold
Publisher
Hainan Medical University
DOI
10.1016/j.apjtb.2015.07.021
Abstract
Objective: To examine the efficacy of 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) iron chelator and green tea extract (GTE) as anti-malarial activity in Plasmodium berghei ( P. berghei) infected mice. Methods: The CM1 (0-100 mg/kg/day) and GTE (0-100 mg (-)-epigallocatechin 3-gallate equivalent/kg/day) were orally administered to P. berghei infected mice for consecutive 4 days. Parasitized red blood cells (PRBC) were enumerated by using Giemsa staining microscopic method. Results: CM1 lowered percentage of PRBC in dose-dependent manner with an ED50 value of 56.91 mg/kg, when compared with pyrimethamine (PYR) (ED50 = 0.76 mg/kg). GTE treatment did not show any inhibition of the malaria parasite growth. In combined treatment, CM1 along with 0.6 mg/kg PYR significantly inhibited the growth of P. berghei in mice while GTE did not enhance the PYR anti-malarial activity. Conclusions: CM1 would be effective per se and synergize with PYR in inhibiting growth of murine malaria parasites, possibly by limiting iron supply from plasma transferrin and host PRBC cytoplasm, and chelating catalytic iron cstitutive in parasites' mitochondrial cytochromes and cytoplasmic ribonucleotide reductase. CM1 would be a promising adjuvant to enhance PYR anti-malarial activity and minimize the drug resistance. © 2015 Hainan Medical University.
Keyword
Antimalarial drug | Green tea | Hydroxypyridinone | iron chelator | Plasmodium
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 3
Funding Sponsor
Thailand Graduate Institute of Science and Technology
License
CC BY-NC-SA
Rights
Wolters Kluwer Medknow Publications
Publication Source
Scopus