-
Inhibitory effect of novel iron chelator, 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) and green tea extract on growth of Plasmodium falciparum
- Back
Metadata
Document Title
Inhibitory effect of novel iron chelator, 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) and green tea extract on growth of Plasmodium falciparum
Author
Thipubon P., Uthaipibull C., Kamchonwongpaisan S., Tipsuwan W., Srichairatanakool S.
Name from Authors Collection
Affiliations
Department of Biochemistry, Faculty of Medicine, Chiang Mai University, 110 Inthawaroros Street, Tambol Sriphum, Amphur Muang Chiang Mai, 50200, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, 12102, Thailand; Division of Biochemistry, School of Medical Science, University of Phayao, Phayao, 56000, Thailand
Type
Article
Source Title
Malaria Journal
ISSN
14752875
Year
2015
Volume
14
Issue
1
Open Access
Gold, Green
Publisher
BioMed Central Ltd.
DOI
10.1186/s12936-015-0910-1
Abstract
Background: Iron is an essential micronutrient required by all living organisms including malaria parasites (Plasmodium spp.) for many biochemical reactions, especially growth and multiplication processes. Therefore, malaria parasite needs to take up the iron from outside or/and inside the parasitized red blood cells (PRBC). Iron chelators are widely used for the treatment of thalassaemia-related iron overload and also inhibit parasite growth at levels that are non-toxic to mammalian cells. Methods: Inhibitory effect of 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) and green tea extract (GTE) on the growth of malaria parasite Plasmodium falciparum was compared with standard chelators including desferrioxamine (DFO), deferiprone (DFP) and deferasirox (DFX). A flow cytometric technique was used to enumerate PRBC stained with SYBR Green I fluorescent dye. The labile iron pool (LIP) was assayed using the calcein-acetoxymethyl fluorescent method. Results: The IC50 values of DFO, GTE, CM1, DFX and DFP against P. falciparum were 14.09, 21.11, 35.14, 44.71 and 58.25 μM, respectively. Importantly, CM1 was more effective in reducing LIP levels in the P. falciparum culture than DFP (p < 0.05). Conclusions: CM1 and GTE exhibit anti-malarial activity. They could interfere with uptake of exogenous iron or deplete the intracellular labile iron pool in malaria parasites, leading to inhibition of their growth. © 2015 Thipubon et al.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Science and Technology Development Agency; Thailand Graduate Institute of Science and Technology; Faculty of Medicine, Chiang Mai University
License
CC BY
Rights
Author
Publication Source
Scopus