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Mechanistic study of intertypic nucleoprotein complex formation and its inhibitory effect toward influenza A virus
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Document Title
Mechanistic study of intertypic nucleoprotein complex formation and its inhibitory effect toward influenza A virus
Author
Narkpuk J., Jaru-Ampornpan P., Subali T., Bertulfo F.C.T., Wongthida P., Jongkaewwattana A.
Name from Authors Collection
Scopus Author ID
55279790400
Affiliations
Virology and Cell Technology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani, Thailand; Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta, Indonesia; Department of Molecular Biology and Biotechnology, University of the Phillipines Los Banos, Laguna, Philippines
Type
Article
Source Title
Virology
ISSN
00426822
Year
2015
Volume
485
Page
104-115
Open Access
Bronze
Publisher
Academic Press Inc.
DOI
10.1016/j.virol.2015.06.032
Abstract
Co-infection of influenza A and B viruses (IAV and IBV) results in marked decreases in IAV replication. Multiple mechanisms have been proposed for this phenomenon. Recently, we reported that IBV nucleoprotein (BNP) alone can suppress IAV replication and proposed an inhibition model in which BNP binds IAV nucleoprotein (ANP) and disrupts IAV polymerase complexes. Here, using mutagenesis and co-immunoprecipitation, we determined the protein motifs mediating the intertypic ANP-BNP complex and showed that it specifically interferes with ANP[U+05F3]s interaction with the PB2 subunit of the IAV polymerase but not with the other subunit PB1. We further demonstrated that BNP only suppresses growth of IAVs but not other RNA viruses. However, different IAV strains display varied sensitivity toward the BNP[U+05F3]s inhibitory effect. Together, our data provide mechanistic insights into intertypic nucleoprotein complex formation and highlight the role of BNP as a potential broad-spectrum anti-IAV agent. © 2015 Published by Elsevier Inc.
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Funding Sponsor
National Science and Technology Development Agency
License
CC BY or CC BY-NC-ND
Rights
Elsevier B.V.
Publication Source
Scopus