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Bacteriophage mediates efficient gene transfer in combination with conventional transfection reagents
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Metadata
Document Title
Bacteriophage mediates efficient gene transfer in combination with conventional transfection reagents
Author
Donnelly A., Yata T., Bentayebi K., Suwan K., Hajitou A.
Name from Authors Collection
Affiliations
Phage Therapy Group, Division of Brain Sciences, Department of Medicine, Imperial College London, Hammersmith Hospital Campus, London, W12 0NN, United Kingdom; National Nanotechnology Center, National Science and Technology Development Agency, 111 Thailand Science ParkPathum Thani 12120, Thailand; Laboratory of Genetics, University of Balearic islands, Valldemossa Road, Palma, 07122, Spain
Type
Article
Source Title
Viruses
ISSN
19994915
Year
2015
Volume
7
Issue
12
Page
6476-6489
Open Access
Gold, Green
Publisher
MDPI AG
DOI
10.3390/v7122951
Abstract
The development of commercially available transfection reagents for gene transfer applications has revolutionized the field of molecular biology and scientific research. However, the challenge remains in ensuring that they are efficient, safe, reproducible and cost effective. Bacteriophage (phage)-based viral vectors have the potential to be utilized for general gene transfer applications within research and industry. Yet, they require adaptations in order to enable them to efficiently enter cells and overcome mammalian cellular barriers, as they infect bacteria only; furthermore, limited progress has been made at increasing their efficiency. The production of a novel hybrid nanocomplex system consisting of two different nanomaterial systems, phage vectors and conventional transfection reagents, could overcome these limitations. Here we demonstrate that the combination of cationic lipids, cationic polymers or calcium phosphate with M13 bacteriophage-derived vectors, engineered to carry a mammalian transgene cassette, resulted in increased cellular attachment, entry and improved transgene expression in human cells. Moreover, addition of a targeting ligand into the nanocomplex system, through genetic engineering of the phage capsid further increased gene expression and was effective in a stable cell line generation application. Overall, this new hybrid nanocomplex system (i) provides enhanced phage-mediated gene transfer; (ii) is applicable for laboratory transfection processes and (iii) shows promise within industry for large-scale gene transfer applications. © 2015 by the authors; licensee MDPI, Basel, Switzerland.
Funding Sponsor
Medical Research Council
License
CC BY
Rights
Author
Publication Source
Scopus