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Carbon nanotag based visual detection of influenza A virus by a lateral flow immunoassay
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Metadata
Document Title
Carbon nanotag based visual detection of influenza A virus by a lateral flow immunoassay
Author
Wiriyachaiporn N., Sirikett H., Maneeprakorn W., Dharakul T.
Name from Authors Collection
Affiliations
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), PathumThani, 12120, Thailand; Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand
Type
Article
Source Title
Microchimica Acta
ISSN
00263672
Year
2017
Volume
184
Issue
6
Page
1827-1835
Open Access
Green
Publisher
Springer-Verlag Wien
DOI
10.1007/s00604-017-2191-6
Abstract
The authors report on a rapid and direct visual test for the detection of influenza A virus using a carbon nanotag based lateral flow assay. Carbon nanoparticles in the form of nanostrings are acting as reporters. As carbon nanotags accumulate in the test zone due to formation of an antibody-antigen-carbon nanotag antibody complex, and this allows for the direct visualization of the analytical signal. Under optimal conditions, influenza A virus can be determined in allantoic fluid inoculated with the virus with a limit of detection of 350 TCID50.mL−1 (i.e., the 50% tissue culture infectious dose). No interference is found for several other tested proteins, and for closely related viruses. Cell lysates containing different seasonal strains of influenza A viruses (including the H1N1 and H3N2 strains) collected from clinical samples were analyzed. It is demonstrated that the method can detect both influenza A viruses without interference by biological matrices. In our perception, this method has a wide potential in that it may be extended to a generally applicable platform for rapid diagnosis influenza A viruses. [Figure not available: see fulltext.]. © 2017, Springer-Verlag Wien.
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Funding Sponsor
National Nanotechnology Center
License
CC BY
Rights
Author
Publication Source
Scopus