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An extreme vertices mixture design approach to optimization of tyrosinase inhibition effects
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Metadata
Document Title
An extreme vertices mixture design approach to optimization of tyrosinase inhibition effects
Author
Wangkananon W., Phuaksaman C., Koobkokkruad T., Natakankitkul S.
Name from Authors Collection
Scopus Author ID
16307188900
Affiliations
Faculty of Engineering, King Mongkut’s University of Technology North Bangkok, Bangkok, 10800, Thailand; National Nanotechnology Center (NANOTEC), National Science and Technology Development AgencyPathumthani 12120, Thailand; Department of Pharmacy Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, 50200, Thailand
Type
Article
Source Title
Engineering Journal
ISSN
01258281
Year
2018
Volume
22
Issue
1
Page
175-185
Open Access
Bronze
Publisher
Chulalongkorn University
DOI
10.4186/ej.2018.22.1.175
Abstract
The objective of this study is to optimize the tyrosinase inhibition effects of three mixtures included Emblica extract, L-Ascorbic acid, and Kojic acid. Tyrosinase is a copper-containing oxidase, which has activity for both catechol and cresol. It is responsible for browning reactions. Mushroom tyrosinase was used in this study as the tyrosinase source and L-DOPA was used to activate the browning activity. The seventeen formulations were conducted by extreme vertices mixture design. To specify the lower and upper limit, prior study for each ingredient was experimented. Ten and thirty-three percentage of inhibition were proposed to specify the lower and upper limit of each ingredient. The result of experiment shown the difference tyrosinase inhibition effects of seventeen formulations. The analysis of variance for result was significant for all pairs and all of components with 92.89 percentage of R-square adjusted. To optimize the best combination, used of response optimization by setting up the target to 80, 85, 90, and 95 percentage of tyrosinase inhibition effect. The optimum combination with 90%inhibition target is 3.1541 mg./ml. of Emblica extract, 0.1420 mg./ml. of L-Ascorbic acid, and 0.0297 mg./ml. of Kojic acid, this formula unlike the formula that was experimented on. © 2018, Chulalongkorn University. All rights reserved.
Funding Sponsor
National Science and Technology Development Agency
License
CC BY-NC-ND
Rights
EJ
Publication Source
Scopus