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A sensitive electrochemical immunosensor based on poly(2-aminobenzylamine) film modified screen-printed carbon electrode for label-free detection of human immunoglobulin G
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Metadata
Document Title
A sensitive electrochemical immunosensor based on poly(2-aminobenzylamine) film modified screen-printed carbon electrode for label-free detection of human immunoglobulin G
Author
Putnin T., Jumpathong W., Laocharoensuk R., Jakmunee J., Ounnunkad K.
Name from Authors Collection
Scopus Author ID
57195305667
Affiliations
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand; The Graduate School, Chiang Mai University, Chiang Mai, Thailand; Department of Chemical Biology, Chulabhorn Graduate Institute, Bangkok, Thailand; Nanostructures and Functional Assembly Laboratory (NFA), National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand; Department of Chemistry, Center of Excellence for Innovation in Chemistry (PERCH-CIC), Faculty of Science, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence in Materials Science and Technology, Chiang Mai University, Chiang Mai, Thailand
Type
Article
Source Title
Artificial Cells, Nanomedicine and Biotechnology
ISSN
21691401
Year
2018
Volume
46
Issue
5
Page
1042-1051
Open Access
Bronze
Publisher
Taylor and Francis Ltd.
DOI
10.1080/21691401.2017.1360322
Abstract
This work focuses on fabricating poly(2-aminobenzylamine)-modified screen-printed carbon electrode as an electrochemical immunosensor for the label-free detection of human immunoglobulin G. To selectively detect immunoglobulin G, the anti-immunoglobulin G antibody with high affinity to immunoglobulin G was covalently linked with the amine group of poly(2-aminobenzylamine) film-deposited screen-printed carbon electrode. The selectivity for immunoglobulin G was subsequently assured by being challenged with redox-active interferences and adventitious adsorption did not significantly interfere the analyte signal. To obviate the use of costly secondary antibody, the [Fe(CN)6]4-/3- redox probe was instead applied to measure the number of human immunoglobulin G through the immunocomplex formation that is quantitatively related to the level of the differential pulse voltammetric current. The resulting immunosensor exhibited good sensitivity with the detection limit of 0.15 ng mL−1, limit of quantitation of 0.50 ng mL−1 and the linear range from 1.0 to 50 ng mL−1. Given those striking analytical performances and the affordability arising from using cheap screen-printed carbon electrode with label-free detection, the immunosensor serves as a promising model for the next-step development of a diagnostic tool. © 2017 Informa UK Limited, trading as Taylor & Francis Group.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Center of Excellence for Learning in Education, Science and Technology; Center of Excellence in Regulatory Science and Innovation; Office of the Higher Education Commission; National Science and Technology Development Agency; Thailand Research Fund; Thailand Graduate Institute of Science and Technology; Faculty of Science, Chiang Mai University
License
CC BY or CC BY-NC-ND
Rights
Taylor & Francis Group
Publication Source
Scopus