-
The gastric sieve of penaeid shrimp species is a sub-micrometer nutrient filter
- Back
Metadata
Document Title
The gastric sieve of penaeid shrimp species is a sub-micrometer nutrient filter
Author
Pattarayingsakul W., Pudgerd A., Munkongwongsiri N., Vanichviriyakit R., Chaijarasphong T., Thitamadee S., Kruangkum T.
Name from Authors Collection
Affiliations
Department of Biotechnology, Faculty of Science, Mahidol University, Rama VI Rd, Bangkok, 10400, Thailand; Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Rama VI Rd, Bangkok, 10400, Thailand; Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd, Bangkok, 10400, Thailand; Division of Anatomy, School of Medical Science, University of Phayao, Muang, Phayao, 56000, Thailand; Aquatic Animal Health Research Team (AQHT), National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Yothi office, Rama VI Rd, Bangkok, 0400, Thailand
Type
Article
Source Title
Journal of Experimental Biology
ISSN
00220949
Year
2019
Volume
222
Issue
10
Open Access
Bronze
Publisher
Company of Biologists Ltd
DOI
10.1242/jeb.199638
Abstract
Unlike that of vertebrates, the penaeid shrimp stomach is of ectodermic origin and is thus covered by a cuticle that is sloughed upon molting. It is composed of two chambers, here called the anterior and posterior stomach chambers, ASC and PSC, respectively. The PSC contains a filtration structure variously called a pyloric filter, filter press, gastric filter or gastric sieve (GS), and the last of these will be used here. The GS resembles an elongated, inverted-V, dome-like, chitinous structure with a midline ridge that is integral to the ventral base of the PSC. The dome surface is covered with a carpet-like layer of minute, comb-like setae bearing laterally branching setulae. This carpet serves as a selective filter that excludes large partially digested food particles but allows smaller particles and soluble materials to enter hepatopancreatic ducts that conduct them into the shrimp hepatopancreas (HP), where further digestion and absorption of nutrients takes place. Although the GS function is well known, its exclusion limit for particulate material has not been clearly defined. Using histological and ultra-structure analysis, we show that the GS sieve pore diameter is approximately 0.2–0.7 µm in size, indicating a size exclusion limit of substantially less than 1 µm. Using fluorescent microbeads, we show that particles of 1 µm diameter could not pass through the GS but that particles of 0.1 µm diameter did pass through to accumulate in longitudinal grooves and move on to the HP, where some were internalized by tubule epithelial cells. We found no significant difference in these sizes between the species Penaeus monodon and Penaeus vannamei or between juveniles and adults in P. vannamei. This information will be of value for the design of particulate feed ingredients such as nutrients, therapeutic drugs and toxin-absorbing materials that may selectively target the stomach, intestine or HP of cultivated shrimp. © 2019. Published by The Company of Biologists Ltd.
Funding Sponsor
Mahidol University; Thailand Research Fund
License
CC BY
Rights
Author
Publication Source
Scopus