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Immobilization-free electrochemical sensor coupled with a graphene-oxide-based aptasensor for glycated albumin detection
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Metadata
Document Title
Immobilization-free electrochemical sensor coupled with a graphene-oxide-based aptasensor for glycated albumin detection
Author
Waiwinya W., Putnin T., Pimalai D., Chawjiraphan W., Sathirapongsasuti N., Japrung D.
Name from Authors Collection
Scopus Author ID
57195305667
Scopus Author ID
57194972179
Affiliations
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani12120, Thailand; Section of Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand
Type
Article
Source Title
Biosensors
ISSN
20796374
Year
2021
Volume
11
Issue
3
Open Access
Gold, Green
Publisher
MDPI
DOI
10.3390/bios11030085
Abstract
An immobilization-free electrochemical sensor coupled with a graphene oxide (GO)-based aptasensor was developed for glycated human serum albumin (GHSA) detection. The concentration of GHSA was monitored by measuring the electrochemical response of free GO and aptamer-bound GO in the presence of glycated albumin; their currents served as the analytical signals. The electrochemical aptasensor exhibited good performance with a base-10 logarithmic scale. The calibration curve was achieved in the range of 0.01–50 µg/mL. The limit of detection (LOD) was 8.70 ng/mL. The developed method was considered a one-drop measurement process because a fabrication step and the probe-immobilization process were not required. This simple sensor offers a cost-effective, rapid, and sensitive detection method, and could be an alternative approach for determination of GHSA levels. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Funding Sponsor
National Science and Technology Development Agency; National Nanotechnology Center
License
CC BY
Rights
Author
Publication Source
Scopus