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Rna sequencing reveals rice genes involved in male reproductive development under temperature alteration
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Metadata
Document Title
Rna sequencing reveals rice genes involved in male reproductive development under temperature alteration
Author
Khlaimongkhon S., Chakhonkaen S., Tongmark K., Sangarwut N., Panyawut N., Wasinanon T., Sikaewtung K., Wanchana S., Mongkolsiriwatana C., Chunwonges J., Muangprom A.
Name from Authors Collection
Scopus Author ID
24467446600
Scopus Author ID
57222568531
Scopus Author ID
56353145600
Scopus Author ID
57194215247
Scopus Author ID
57194206928
Affiliations
Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Kamphaeng Saen, 73140, Thailand; Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok, 10900, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand; Division of Genetics, Faculty of Liberal Arts and Science, Kasetsart University, Nakhon Pathom, 73140, Thailand; Department of Horticulture, Faculty of Agriculture, Kasetsart University, Nakhon Pathom, 73140, Thailand
Type
Article
Source Title
Plants
ISSN
22237747
Year
2021
Volume
10
Issue
4
Open Access
Gold, Green
Publisher
MDPI AG
DOI
10.3390/plants10040663
Abstract
Rice (Oryza sativa L.) is one of the most important food crops, providing food for nearly half of the world population. Rice grain yields are affected by temperature changes. Temperature stresses, both low and high, affect male reproductive development, resulting in yield reduction. Thermosensitive genic male sterility (TGMS) rice is sterile at high temperature and fertile at low temperature conditions, facilitating hybrid production, and is a good model to study effects of temperatures on male development. Semithin sections of the anthers of a TGMS rice line under low (fertile) and high (sterile) temperature conditions showed differences starting from the dyad stage, suggesting that genes involved in male development play a role during postmeiotic microspore development. Using RNA sequencing (RNA-Seq), transcriptional profiling of TGMS rice panicles at the dyad stage revealed 232 genes showing differential expression (DEGs) in a sterile, compared to a fertile, condition. Using qRT-PCR to study expression of 20 selected DEGs using panicles of TGMS and wild type rice plants grown under low and high temperature conditions, revealed that six out of the 20 selected genes may be unique to TGMS, while the other 14 genes showed common responses to temperatures in both TGMS and wild-type rice plants. The results presented here would be useful for further investigation into molecular mechanisms controlling TGMS and rice responses to temperature alteration. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Keyword
Anther | Rice | RNA-seq | TGMS | Transcriptome
Funding Sponsor
Office of the Higher Education Commission; National Science and Technology Development Agency; Agricultural Research Development Agency; Ministry of Higher Education, Science, Research and Innovation, Thailand
License
CC BY
Rights
Author
Publication Source
Scopus