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Peptidomics analysis of virulent peptides involved in streptococcus suis pathogenesis
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Metadata
Document Title
Peptidomics analysis of virulent peptides involved in streptococcus suis pathogenesis
Author
Chaiden C., Jaresitthikunchai J., Phaonakrop N., Roytrakul S., Kerdsin A., Nuanualsuwan S.
Name from Authors Collection
Affiliations
Department of Veterinary Public Health, Faculty of Veterinary Sciences, Chulalongkorn University, Bangkok, 10330, Thailand; Functional Proteomics Technology Laboratory, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology for Development Agency, Pathum Thani, 12120, Thailand; Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, 47000, Thailand; Food Risk Hub, Research Unit of Chulalongkorn University, Bangkok, 10330, Thailand
Type
Article
Source Title
Animals
ISSN
20762615
Year
2021
Volume
11
Issue
9
Open Access
Gold, Green
Publisher
MDPI AG
DOI
10.3390/ani11092480
Abstract
Streptococcus suis (S. suis) is a zoonotic pathogen causing severe streptococcal disease worldwide. S. suis infections in pigs and humans are frequently associated with the virulent S. suis serotype 2 (SS2). Though various virulence factors of S. suis have been proposed, most of them were not essentially accounted for in the experimental infections. In the present study, we compared the peptidomes of highly virulent SS2 and SS14 in humans, the swine causative serotypes SS7 and SS9, and the rarely reported serotypes SS25 and SS27, and they were cultured in a specified culture medium containing whole blood to simulate their natural host environment. LC-MS/MS could identify 22 unique peptides expressed in the six S. suis serotypes. Under the host-simulated environment, peptides from the ABC-type phosphate transport system (SSU05_1106) and 30S ribosomal protein S2 (rpsB) were detected in the peptidome of virulent SS2 and SS14. Therefore, we suggest that these two proteins or their derived peptides might be involved in the survival of S. suis when simulated with a blood environment. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Funding Sponsor
Chulalongkorn University
License
CC BY
Rights
Author
Publication Source
Scopus