-
Expressed sequence tag analysis for identification and characterization of sex-related genes in the giant tiger shrimp Penaeus monodon
- Back
Metadata
Document Title
Expressed sequence tag analysis for identification and characterization of sex-related genes in the giant tiger shrimp Penaeus monodon
Author
Preechaphol R., Leelatanawit R., Sittikankeaw K., Klinbunga S., Khamnamtong B., Puanglarp N., Menasveta P.
Name from Authors Collection
Scopus Author ID
19337937700
Affiliations
Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence for Marine Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Aquatic Molecular Genetics and Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand; Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
Type
Article
Source Title
Journal of Biochemistry and Molecular Biology
ISSN
12258687
Year
2007
Volume
40
Issue
4
Page
501-510
Open Access
Bronze
Publisher
The Biochemical Society of the Republic of Korea
DOI
10.5483/bmbrep.2007.40.4.501
Abstract
Sex-related genes expressed in vitellogenic ovaries of the giant tiger shrimp, Penaeus monodon, were identified by an EST approach. A total of 1051 clones were unidirectionally sequenced from the 5 terminus. Nucleotide sequences of 743 EST (70.7%) significantly matched known genes previously deposited in the GenBank (E-value <10-4) whereas 308 ESTs (29.3%) were regarded as newly unidentified transcripts (E-value >10-4). A total of 559 transcripts (87 contigs and 472 singletons) were obtained. Thrombospondin (TSP) and peritrophin (79 and 87 clones accounting for 7.5 and 8.3% of clones sequenced, respectively) predominated among characterized transcripts. Several full length transcripts (e.g. cyclophilin, profillin and thioredoxin peroxidase) were also isolated. A gene homologue encoding chromobox protein (PMCBX, ORF of 567 nucleotides encoding a protein of 188 amino acids) which is recognized as a new member of the HP1 family was identified. Expression patterns of 14 of 25 sex-related gene homologues in ovaries and testes of P. monodon broodstock were examined by RT-PCR. Female sterile and ovarian lipoprotein receptor homologues were only expressed in ovaries whereas the remaining transcripts except disulfide isomerase related P5 precursor and adenine nucleotide translocator 2 were higher expressed in ovaries than testes of P. monodon broodstock. A homologue of ubiquitin specific proteinase 9, X chromosome (Usp9X) revealed a preferential expression level in ovaries than testes of broodstock-sized P. monodon (N = 13 and 11, P <0.05) but was only expressed in ovaries of 4-month-old shrimp (N = 5 for each sex).
Keyword
EST | Penaeus monodon | RT-PCR | Sex-specific markers | Shrimp
License
Public Nuri
Rights
Korea Institute of Science and Technology Information https://www.kisti.re.kr/pageView/521?t=1648956401355
Publication Source
Scopus