-
Effect of Variation in hemorheology between human and animal blood on the binding efficacy of vascular-targeted carriers
- Back
Document Title
Effect of Variation in hemorheology between human and animal blood on the binding efficacy of vascular-targeted carriers
Author
Namdee K., Carrasco-Teja M., Fish M.B., Charoenphol P., Eniola-Adefeso O.
Name from Authors Collection
Affiliations
Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, United States; National Nanotechnology Center, National Science and Technology Development Agency, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand
Type
Article
Source Title
Scientific Reports
ISSN
20452322
Year
2015
Volume
5
Open Access
All Open Access, Gold, Green
Publisher
Nature Publishing Group
DOI
10.1038/srep11631
Format
Abstract
Animal models are extensively used to evaluate the in vivo functionality of novel drug delivery systems (DDS). However, many variations likely exist in vivo between the animals and human physiological environment that significantly alter results obtained with animal models relative to human system. To date, it is not clear if the variation in hemorheology and hemodynamics between common animal and human models affect the functionality of DDS. This study investigates the role of hemorheology of humans and various animal models in dictating the binding efficiency of model vascular-targeted carriers (VTCs) to the wall in physiological blood flows. Specifically, the adhesion of sLe A -coated nano- and micro-spheres to inflamed endothelial cells monolayers were conducted via a parallel plate flow chamber assay with steady and disturbed red blood cells (RBCs)-in-buffer and whole blood flows of common animal models. Our results suggest that the ratio of carrier size to RBC size dictate particle binding in blood flow. Additionally, the presence of white blood cells affects the trend of particle adhesion depending on the animal species. Overall, this work sheds light on some deviation in VTC vascular wall interaction results obtained with in vivo animal experimentation from expected outcome and efficiency in vivo in human.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Science Foundation; National Institutes of Health
License
N/A
Rights
N/A
Publication Source
Scopus