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Antibody biosensors for the measurement and characterization of soluble CD147 molecules
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Document Title
Antibody biosensors for the measurement and characterization of soluble CD147 molecules
Author
Laopajon W., Takheaw N., Khummuang S., Kampoun T., Cheunsirikulchai K., Kasinrerk W., Pata S.
Name from Authors Collection
Affiliations
Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand; Biomedical Technology Research Center, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at the Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand
Type
Article
Source Title
Asian Pacific Journal of Allergy and Immunology
ISSN
0125877X
Year
2018
Volume
36
Issue
3
Page
191-200
Open Access
All Open Access, Bronze
Publisher
Allergy and Immunology Society of Thailand
DOI
10.12932/AP-150217-0022
Format
Abstract
Background: Soluble CD147 (sCD147) is the shed form of membrane-bound CD147, which is involved in the regulation of cellular functions. The presence of sCD147 in body fluids is associated with several diseases. Objective: In this study, we aimed to establish antibody (Ab) biosensors for the simultaneous differential detection of thgeneral and truncated forms of sCD147. Method: By combining biolayer interferometry technology (BLItz) and different anti-CD147 monoclonal antibodie(mAbs) specific to different extracellular domains of the CD147 molecule, Ab-based biosensors were established to rapidlmeasure and characterize sCD147 isoforms. Results: Two types of Ab-biosensors, desginated the single Ab-biosensor and double Ab-biosensor, were established for the measurment and characterization of sCD147 isoforms. For the single Ab-biosensor system, monoclonal antibodies specific for CD147 domain 1 (D1) or domain 2 (D2) were immobilized on the sensor tips and used for the quantification of sCD147 using a BLItz optical interferometric biosensor. For the double Ab-biosensor system, following the single Ab-biosensor step, secondary anti-CD147 mAbs specific for each domain of the CD147 molecule were added and monitored by a BLItz biosensor. By combining the results obtained from the single Ab-and double Ab-biosensors, sCD147 isoforms including the general form (D1 linked to D2) and the truncated forms (sCD147 containing D1 or D2) could be determined. Conclusions: This method may be a beneficial tool for the determination of sCD147 isoforms for disease diagnosis prognosis as well as for the definition of the cellular mechanisms of the immune system. © 2018, Allergy and Immunology Society of Thailand. All rights reserved.
Keyword
Biosensor | CD147 | Isoform | Monoclonal antibody | Soluble CD147
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Chiang Mai University; Thailand Research Fund
License
CC BY-NC
Rights
Author
Publication Source
Scopus