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Identification and characterization of glycoproteins during oil palm somatic embryogenesis
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Document Title
Identification and characterization of glycoproteins during oil palm somatic embryogenesis
Author
Aroonluk S., Roytrakul S., Yingchutrakul Y., Kittisenachai S., Jantasuriyarat C.
Name from Authors Collection
Scopus Author ID
55043184800
Affiliations
Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), PathumThani, 12120, Thailand; Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University (CASTNAR, NRU-KU), Bangkok, 10900, Thailand
Type
Article
Source Title
Agriculture and Natural Resources
ISSN
24681458
Year
2018
Volume
52
Issue
5
Page
430-438
Open Access
All Open Access, Hybrid Gold
Publisher
Elsevier B.V.
DOI
10.1016/j.anres.2018.11.011
Format
Abstract
The objective of this experiment was to characterize differentially expressed glycoprotein in a somatic embryogenesis process during oil palm tissue culture. Embryogenic callus in the somatic embryo acquisition stages (globular, torpedo and cotyledonary) and oil palm plantlets were collected to extract total protein and isolated glycoprotein using a concanavalin A N-linked affinity column. Tryptic glycoproteins were isolated using nano-liquid chromatography-tandem mass spectrometry. In total, 383 glycoproteins were identified and analyzed. The percentages of existence of glycoprotein were observed, from highest to lowest amounts, in the metabolic process, binding and nuclear components, respectively. Different glycoproteins were involved in membrane trafficking machinery, signaling to stress and in hormonal and environmental response for plant growth and development. PREDICTED: protein TIC 40, chloroplastic-like was expressed in the globular, torpedo and plantlet stages. The protein can be further developed into a glycoprotein biomarker, as a biological indicator of the somatic embryo maturation stage. This study should assist deeper understanding of the important role of glycoprotein specificity in somatic embryogenesis that controls plant cell response and growth development during oil palm tissue culture. © 2018 Kasetsart University
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Research Council of Thailand; Kasetsart University Research and Development Institute; National Center for Genetic Engineering and Biotechnology
License
CC BY-NC-ND
Rights
Author
Publication Source
Scopus