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Long non-coding RNA H19 enhances cell proliferation and anchorage-independent growth of cervical cancer cell lines
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Document Title
Long non-coding RNA H19 enhances cell proliferation and anchorage-independent growth of cervical cancer cell lines
Author
Iempridee T.
Name from Authors Collection
Affiliations
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathum Thani, 12120, Thailand
Type
Article
Source Title
Experimental Biology and Medicine
ISSN
15353702
Year
2017
Volume
242
Issue
2
Page
184-193
Open Access
All Open Access, Green
Publisher
SAGE Publications Inc.
DOI
10.1177/1535370216670542
Format
Abstract
Long non-coding RNA H19 is aberrantly expressed in multiple malignancies and its expression levels correlate with recurrence, metastasis, and patient survival. Despite numerous reports documenting the role of H19 in carcinogenesis, its contribution to cervical cancer development is still largely unknown. In this study, I observed that H19 expression was elevated in cervical cancer cell lines and could be detected in extracellular vesicles in the culture medium. In addition, I demonstrated, by overexpression and knockdown experiments, that H19 promoted cell proliferation and multicellular tumor spheroid formation without significantly affecting apoptosis and cell migration. Finally, treatment with transforming growth factor beta and hypoxia-mimetic CoCl2 could modulate H19 levels in a cell line-specific manner. These findings indicate that H19 promotes both anchorage-specific and -independent growth of cervical cancer cell lines and may serve as a potential target for cancer diagnosis and therapy. © 2016, © 2016 by the Society for Experimental Biology and Medicine.
Keyword
cervical cancer | extracellular vesicles | H19 | hypoxia | lncRNA | transforming growth factor beta
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Nanotechnology Center
License
N/A
Rights
N/A
Publication Source
Scopus