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Interaction of CD99 and its ligand upregulates IL-6 and TNF-alpha upon T cell activation
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Document Title
Interaction of CD99 and its ligand upregulates IL-6 and TNF-alpha upon T cell activation
Author
Takheaw N, Earwong P, Laopajon W, Pata S, Kasinrerk W
Name from Authors Collection
Affiliations
Chiang Mai University; Chiang Mai University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
PLOS ONE
ISSN
1932-6203
Year
2019
Volume
14
Issue
1
Open Access
Green Submitted, Green Published, gold
Publisher
PUBLIC LIBRARY SCIENCE
DOI
10.1371/journal.pone.0217393
Format
Abstract
CD99 has been reported to be involved in T cell regulation. CD99 ligand involvement in the regulation of T cell activation has been postulated. In this study, recombinant CD99 proteins were produced and used as a tool for determining the role of CD99 and its ligand interaction. Recombinant CD99 proteins induced the upregulation of IL-6 and TNF-alpha expression, but not IFN-gamma, in anti-CD3 monoclonal antibody activated T cells. The cytokine alteration was not observed in unstimulated T cells indicating the cytokine upregulation required the signal from T cell activation. The upregulation of IL-6 and TNF-alpha was, in addition, observed in CD3(-) mononuclear cell population including monocytes and NK cells. The recombinant CD99 proteins, however, did not affect either CD25, CD69 or MHC class II expression or T cell proliferation, upon T cell activation. The CD99 ligands were demonstrated to be expressed on monocytes, NK cells and dendritic cells, but not on B and T cells. Our results indicated the presence of CD99 ligands on leukocyte surface. Interaction between CD99 and its ligands involves the regulation of cytokine production.
Funding Sponsor
TRF [RTA5980007]; Thailand Office of Higher Education Commission [MRG6080269, MRG6180253]; Chiang Mai University under the CMU Center of Excellence Project; Graduate school, Chiang Mai University; Royal Golden Jubilee Ph.D. program [PHD/0121/2557]
License
CC-BY
Rights
Authors
Publication Source
WOS