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Sensitivity Validation of EWOD Devices for Diagnosis of Early Mortality Syndrome (EMS) in Shrimp Using Colorimetric LAMP-XO Technique
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Document Title
Sensitivity Validation of EWOD Devices for Diagnosis of Early Mortality Syndrome (EMS) in Shrimp Using Colorimetric LAMP-XO Technique
Author
Sukthang K, Kampeera J, Sriprachuabwong C, Kiatpathomchai W, Pengwang E, Tuantranont A, Wechsatol W
Name from Authors Collection
Scopus Author ID
8532633300
Affiliations
King Mongkuts University of Technology Thonburi; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); National Science & Technology Development Agency - Thailand; King Mongkuts University of Technology Thonburi
Type
Article
Source Title
SENSORS
Year
2021
Volume
21
Open Access
gold, Green Published
Publisher
MDPI
DOI
10.3390/s21093126
Format
Abstract
Electrowetting-on-dielectric (EWOD) is a microfluidic technology used for manipulating liquid droplets at microliter to nanoliter scale. EWOD has the ability to facilitate the accurate manipulation of liquid droplets, i.e., transporting, dispensing, splitting, and mixing. In this work, EWOD fabrication with suitable and affordable materials is proposed for creating EWOD lab-on-a-chip platforms. The EWOD platforms are applied for the diagnosis of early mortality syndrome (EMS) in shrimp by utilizing the colorimetric loop-mediated isothermal amplification method with pH-sensitive xylenol orange (LAMP-XO) diagnosis technique. The qualitative sensitivity is observed by comparing the limit of detection (LOD) while performing the LAMP-XO diagnosis test on the proposed lab-on-a-chip EWOD platform, alongside standard LAMP laboratory tests. The comparison results confirm the reliability of EMS diagnosis on the EWOD platform with qualitative sensitivity for detecting the EMS DNA plasmid concentration at 10(2) copies in a similar manner to the common LAMP diagnosis tests.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Thailand Research Fund (TRF) [RSA5680026]; King Mongkut's University of Technology; Thonburi research fund; Thailand Graduate Institute of Science and Technology (TGIST)
License
CC-BY
Rights
Authors
Publication Source
WOS