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Proteomics analysis of serum protein patterns in duck during aflatoxin B1 exposure
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Document Title
Proteomics analysis of serum protein patterns in duck during aflatoxin B1 exposure
Author
Tansakul N, Rattanasrisomporn J, Roytrakul S
Name from Authors Collection
Affiliations
Kasetsart University; Kasetsart University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
VETERINARY WORLD
ISSN
0972-8988
Year
2019
Volume
12
Issue
2
Open Access
Green Published, gold
Publisher
VETERINARY WORLD
DOI
10.14202/vetworld.2019.1499-1505
Format
Abstract
Background and Aim: Unlike the already well-documented human serum proteome, there are still limitations regarding analyzing and interpreting the various physiological changes and disease states of the serum proteomes found in duck. Serum proteome in duck under the condition of mycotoxin contamination in feed has not yet been examined. This study aimed to introduce the characterization of the circulating proteomes in duck serum during exposure to aflatoxin B1 (AFB1). Materials and Methods: Duck serum samples were collected from four experimental groups, gel-based mass spectrometry was then applied, and finally, 445 proteins were identified in pulled serum sample. Results: Among these 445 proteins, 377 were present in at least one group from all. There were 35 proteins which were expressed when the duck was exposed to AFB 1. The protein library that allows the identification of a large number of different proteins in duck serum will be enhanced by the addition of these peptide spectral data. It is noteworthy that chromodomain-helicase-DNA-binding protein 7 (CHD7) [Gallus gallus] was up-regulated in the group with the highest AFB1 contamination. Conclusion: CHD7 protein might be somehow relative to aflatoxicosis in the duck that causes poor performance and economic loss. Moreover, other proteins present in duck serum were also added in the protein library.
Keyword
aflatoxin B1 | duck | Proteomics | scrum
Funding Sponsor
Thailand Research Fund [TRF-MRG56]
License
CC-BY
Rights
Authors
Publication Source
WOS