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A novel recombinant javanicin with dual antifungal and anti-proliferative activities
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Document Title
A novel recombinant javanicin with dual antifungal and anti-proliferative activities
Author
Orrapin S, Intorasoot A, Roytrakul S, Dechsupa N, Kantapan J, Onphat Y, Srimek C, Tharinjaroen CS, Anukool U, Butr-Indr B, Phunpae P, Intorasoot S
Name from Authors Collection
Affiliations
Chiang Mai University; Chiang Mai University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Chiang Mai University; Chiang Mai University
Type
Article
Source Title
SCIENTIFIC REPORTS
ISSN
2045-2322
Year
2019
Volume
9
Issue
2
Open Access
Green Published, gold
Publisher
NATURE PUBLISHING GROUP
DOI
10.1038/s41598-019-55044-7
Format
Abstract
Resistance to common drugs by microorganisms and cancers has become a major issue in modern healthcare, increasing the number of deaths worldwide. Novel therapeutic agents with a higher efficiency and less side effects for the treatment of certain diseases are urgently needed. Plant defensins have an integral role in a hosts' immune system and are attractive candidates for combatting drug-resistant microorganisms. Interestingly, some of these defensins also showed great potential due to their cytotoxic activity toward cancer cells. In this study, a defensin encoding gene was isolated from five legume seeds using 3' rapid amplification of cDNA ends (3' RACE) with degenerate primers and cDNA cloning strategies. Bioinformatic tools were used for in silico identification and the characterization of new sequences. To study the functional characteristics of these unique defensins, the gene encoded for Sesbania-javanica defensin, designated as javanicin, was cloned into pTXB-1 plasmid and expressed in the Escherichia coli Origami 2 (DE3) strain. Under optimized conditions, a 34-kDa javanicin-intein fusion protein was expressed and approximately 2.5-3.5 mg/L of soluble recombinant javanicin was successfully extracted with over 90% purity. Recombinant javanicin displayed antifungal properties against human pathogenic fungi, including resistant strains, as well as cytotoxic activities toward the human breast cancer cell lines, MCF-7 & MDA-MB-231. Recombinant javanicin holds great promise as a novel therapeutic agent for further medical applications.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Thailand Research and Researcher for Industry (RRi) [PHD5910039]; Chiang Mai University
License
CC BY
Rights
Authors
Publication Source
WOS