Abstract
A novel actinomycete, A-T 8343(T) was isolated from a moist evergreen forest soil sample collected in the Trat Province, Thailand. Based on 16S rRNA gene sequence analysis, strain A-T 83431 belonged to the genus Sinosporangium and was closely related to Sinosporangium siamense A-T 1946(T) (98.81 %) and Sinosporangium album 6014(T) (98.54 %). The DNA-DNA relatedness values were 21.8-27% with S. siamense A-T 1946(T) and 31.1-31.9% with S. album 6014(T), which were significantly below 70%. The result differentiated A-T 8343(T) from the closest species. The organism developed spherical sporangia containing non-motile spores on aerial mycelia. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained rhamnose, ribose, madurose and glucose. The predominant menaquinones were MK-9(H-2) and MK-9(H-4). The diagnostic phospholipids were phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyl-phosphatidylethanolamine, diphosphatidylglycerol, lysophosphatidylethanolamine, N-acetylglucosamine-containing phospholipids, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol-mannosides, aminophosphoglycolipid and one unknown phospholipid. The major cellular fatty acids were saturated C-16:0, iso C-16:0, unsaturated C-16:1 and C-18:1. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the new isolate is proposed as a representative novel species of the genus Sinosporangium to be named Sinosporangium fuscum sp. nov. The type strain is A-T 8343(T) (=BCC 52770(T)=NBRC 109516(T)).
