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Pattern Recognition Protein Binds to Lipopolysaccharide and beta-1,3-Glucan and Activates Shrimp Prophenoloxidase System
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Document Title
Pattern Recognition Protein Binds to Lipopolysaccharide and beta-1,3-Glucan and Activates Shrimp Prophenoloxidase System
Author
Amparyup P, Sutthangkul J, Charoensapsri W, Tassanakajon A
Name from Authors Collection
Affiliations
Chulalongkorn University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
JOURNAL OF BIOLOGICAL CHEMISTRY
Year
2012
Volume
287
Issue
2
Open Access
hybrid, Green Published
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI
10.1074/jbc.M111.294744
Format
Abstract
The prophenoloxidase (proPO) system is activated upon recognition of pathogens by pattern recognition proteins (PRPs), including a lipopolysaccharide-and beta-1,3-glucan-bindingprotein (LGBP). However, shrimp LGBPs that are involved in the proPO system have yet to be clarified. Here, we focus on characterizing the role of a Penaeus monodon LGBP (PmLGBP) in the proPO system. We found that PmLGBP transcripts are expressed primarily in the hemocytes and are increased at 24 h after pathogenic bacterium Vibrio harveyi challenge. The binding studies carried out using ELISA indicated that recombinant (r) PmLGBP binds to beta-1,3-glucan and LPS with a dissociation constant of 6.86 x 10(-7) M and 3.55 x 10(-7) M, respectively. Furthermore, we found that rPmLGBP could enhance the phenoloxidase (PO) activity of hemocyte suspensions in the presence of LPS or beta-1,3-glucan. Using dsRNA interference-mediated gene silencing assay, we further demonstrated that knockdown of PmLGBP in shrimp in vivo significantly decreased the PmLGBP transcript level but had no effect on the expression of the other immune genes tested, including shrimp antimicrobial peptides (AMPs). However, suppression of proPO expression down-regulated PmLGBP, proPO-activating enzyme (PmPPAE2), and AMPs (penaeidin and crustin). Such PmLGBP down-regulated shrimp showed significantly decreased total PO activity. We conclude that PmLGBP functions as a pattern recognition protein for LPS and beta-1,3-glucan in the shrimp proPO activating system.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Thailand Research Fund; Thailand National Center for Genetic Engineering and Biotechnology (BIOTEC); Higher Education Research promotion and National Research University of Thailand; Office of the Higher Education Commission [FW 643A]; National Research University of Commission for Higher Education; Ratchada-phiseksomphot Endowment Fund; Royal Golden Jubilee Ph. D. Program; NSTDA [SP52-BT3]
Publication Source
WOS