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Identification of Thai isolates assigned to the genus Gluconobacter based on 16S-23S rDNA ITS restriction analysis
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Document Title
Identification of Thai isolates assigned to the genus Gluconobacter based on 16S-23S rDNA ITS restriction analysis
Author
Huong VTL, Malimas T, Yukphan P, Potacharoen W, Tanasupawat S, Loan LTT, Tanticharoen M, Yamada Y
Name from Authors Collection
Affiliations
National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Chulalongkorn University
Type
Article
Source Title
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
ISSN
0022-1260
Year
2007
Volume
53
Issue
2
Page
133-142
Open Access
Bronze
Publisher
MICROBIOL RES FOUNDATION
DOI
10.2323/jgam.53.133
Format
Abstract
Forty-four Thai isolates phenotypically assigned to the genus Gluconobacter were examined for 16S-23S rDNA ITS restriction analysis by Mboll and Sdul (=Bsp1286I) digestions. The Thai isolates tested were divided into seven groups: Group I for fourteen isolates, Group IX for one isolate, Group X for two isolates, Group V-2 for four isolates, Group XI for three isolates, Group IV for one isolate, and Group III for nineteen isolates. There were no isolates of either Group 11 or Group V-1 that were identified as G. cerinus. The isolates of Group III, Group IV, and Group XI were subjected to an additional 16S-23S rDNA ITS restriction analysis by Avail, Taql, BsoBl, and BstNl digestions. The isolates of Group III were divided into three groups and two subgroups: Group III-2 for five isolates, Group III-6 for two isolates, and Group III-4, which was divided into two subgroups, Subgroup III-4a for four isolates and Subgroup III-4b for eight isolates. The fourteen isolates of Group I were identified as G. oxydans, and the two isolates of Group X were temporarily identified as G. oxydans. The five isolates of Group III-2 and the one isolate of Group IV were identified as G. frateurii. The remaining twenty-two isolates of Group V-2, Group III-4, Group III-6, Group IX, and Group XI were not identified but are candidates for several new species.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
License
N/A
Rights
N/A
Publication Source
WOS