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Complete Genome Sequence of Virulence-Enhancing Siphophage VHS1 from Vibrio harveyi
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Document Title
Complete Genome Sequence of Virulence-Enhancing Siphophage VHS1 from Vibrio harveyi
Author
Khemayan K, Prachumwat A, Sonthayanon B, Intaraprasong A, Sriurairatana S, Flegel TW
Name from Authors Collection
Affiliations
Mahidol University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN
0099-2240
Year
2012
Volume
78
Issue
8
Page
2790-2796
Open Access
Green Published
Publisher
AMER SOC MICROBIOLOGY
DOI
10.1128/AEM.05929-11
Format
Abstract
Vibrio harveyi siphophage 1 (VHS1) is a tailed phage with an icosahedral head of approximately 66 nm in diameter and an unornamented, flexible tail of approximately 153 nm in length. When Vibrio harveyi 1114GL is lysogenized with VHS1, its virulence for the black tiger shrimp (Penaeus monodon) increases by more than 100 times, and this coincides with production of a toxin(s) associated with shrimp hemocyte agglutination. Curiously, the lysogen does not show increased virulence for the whiteleg shrimp (Penaeus [Litopenaeus] vannamei). Here we present and annotate the complete, circular genome of VHS1 (81,509 kbp; GenBank accession number JF713456). By software analysis, the genome contains 125 putative open reading frames (ORFs), all of which appear to be located on the same DNA strand, similar to the case for many other bacteriophages. Most of the putative ORFs show no significant homology to known sequences in GenBank. Notable exceptions are ORFs for a putative DNA polymerase and putative phage structural proteins, including a portal protein, a phage tail tape measure protein, and a phage head protein. The last protein was identified as a component of the species-specific toxin mixture described above as being associated with agglutination of hemocytes from P. monodon.
Funding Sponsor
Mahidol University; Thai National Center for Genetic Engineering and Biotechnology (BIOTEC); Center of Excellence on Agricultural Biotechnology, Science and Technology Postgraduate Education and Research Development Office, Office of Higher Education Commission, Ministry of Education [AG-GIO/PERDO-CHE]; Alltech Inc., Nicholasville, KY
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