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Phosphoproteome analysis reveals chitosan-induced resistance to osmotic stress in rice (Oryza sativa L.) seedlings
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Document Title
Phosphoproteome analysis reveals chitosan-induced resistance to osmotic stress in rice (Oryza sativa L.) seedlings
Author
Pongprayoon W, Maksup S, Phaonakrop N, Jaresitthikunchai J, Uawisetwathana U, Panya A, Roytrakul S
Name from Authors Collection
Affiliations
Silpakorn University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
JOURNAL OF PLANT INTERACTIONS
ISSN
1742-9145
Year
2022
Volume
17
Issue
1
Page
894-910
Open Access
gold
Publisher
TAYLOR & FRANCIS LTD
DOI
10.1080/17429145.2022.2114556
Format
Abstract
This study sought to identify the mechanism underlying the response to chitosan at the posttranslational level. Khao Dawk Mali 105 seeds were soaked in 40 mg l(-1) of chitosan, and leaves of 2- and 4-week-old seedlings were sprayed with chitosan before starting osmotic stress conditions. Chitosan induced resistance to osmotic stress by enhancing shoot fresh and dry weights and maintained increased photosynthetic pigments. Leaf phosphoproteomes were examined using gel-free LC-MS/MS. Of the 60 phosphoproteins showed a significant difference in protein expressions under osmotically-stressed plants treated with chitosan. More than 40% of the phosphoproteins involved in signaling pathways, including OsCML12 calmodulin-related calcium sensor protein, ubiquitin carboxyl-terminal hydrolase 15, U-box domain-containing protein 45, HEAT repeat family protein, BRCA1 C terminus domain-containing protein, pectinesterase, protein kinase domain-containing protein, and receptor-like protein kinase. Chitosan enhanced rice seedling growth and drought resistance via multiple complex networks, including metabolism, transport, transcription, and signaling under osmotic stress.
Keyword
Chitosan | ESI-LC-MS/MS | Khao Dawk Mali 105 | Osmotic stress | Phosphoproteome | Rice
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Funding Sponsor
Research Fund for DPST Graduate with First Placement [030/2558]; Development and Promotion of Science and Technology Talents Project (DPST); Institute for the Promotion of Teaching Science and Technology (IPST), Thailand
License
CC-BY
Rights
Authors
Publication Source
WOS
Document
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Phosphoproteome analysis reveals chitosan-induced resistance to osmotic stress in rice (Oryza sativa L.) seedlingsDownload