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Serum protein expression in Equine Glandular Gastric Disease (EGGD) induced by phenylbutazone
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Document Title
Serum protein expression in Equine Glandular Gastric Disease (EGGD) induced by phenylbutazone
Author
Tesena P, Yingchutrakul Y, Roytrakul S, Wongtawan T, Angkanaporn K
Name from Authors Collection
Affiliations
Chulalongkorn University; Mahidol University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Mahidol University; Chulalongkorn University
Type
Article
Source Title
JOURNAL OF VETERINARY MEDICAL SCIENCE
ISSN
0916-7250
Year
2019
Volume
81
Issue
3
Page
418-424
Open Access
gold, Green Published, Green Submitted
Publisher
JAPAN SOC VET SCI
DOI
10.1292/jvms.18-0679
Format
Abstract
Equine Glandular Gastric Disease (EGGD) is a common disease in sport horses. This disease might be associated with usage of nonsteroidal anti-inflammatory drugs (NSAIDs) for treating inflammatory diseases. Although gastroscopy has been an effective method for diagnosis, but a less invasive, and inexpensive method is preferred. This study used proteomic technology to identify candidate serum proteins that might be used as markers of NSAIDs induced EGGD. Five Thoroughbred horses were given high doses of NSAID, phenylbutazone to treat lameness. The experiment was divided into three periods: (i) Pre-EGGD period, (ii) during EGGD period, and (iii) Post-EGGD period. Gastroscopy were used to diagnose EGGD, serum was collected to perform gel electrophoresis (1D SDS-PAGE) and mass spectrometry (LC-MS) in order to identify serum proteins in each group. The candidate serum proteins were computationally predicted for the interaction between phenylbutazone and proteins, tissue specific expression, and association to gastric ulceration. After EGGD induction, all horses showed clinical signs of colic with marked congestion and erosion appearing in the mucosa of the glandular stomach whereas no change was observed in the mucosa of non-glandular stomach. Our proteomic results identified 14 proteins that might be used as EGGD markers. These proteins were highly expressed in the glandular stomach and some proteins were associated with phenylbutazone or ulcer development. However, confirmation of these candidate marker proteins is required with specific antibodies in the larger horse population before they can be considered for application in the field.
Keyword
EGGD | horse | marker | NSAIDs | Proteomics
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Funding Sponsor
Research Scholarships, CU Graduate School Thesis Grant, Graduate School, Chulalongkorn University, Faculty of Veterinary Science Mahidol University, Faculty of Veterinary Science Chulalongkorn University; Proteomics Research Laboratory, Genome Technology Research Unit, National Center for Genetics Engineering and Biotechnology, Faculty of Veterinary Science, Mahidol University
Publication Source
WOS