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Investigation on the Prevalence of Canine Microfilaremia in Thailand Using a Novel Microfluidic Device in Combination with Real-Time PCR
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Metadata
Document Title
Investigation on the Prevalence of Canine Microfilaremia in Thailand Using a Novel Microfluidic Device in Combination with Real-Time PCR
Author
Loymek S, Phuakrod A, Zaelai K, Sripumkhai W, Vongjaroensanti P, Wongkamchai S
Name from Authors Collection
Affiliations
Ministry of Public Health - Thailand; Mahidol University; Mahidol University; National Science & Technology Development Agency - Thailand; National Electronics & Computer Technology Center (NECTEC)
Type
Article
Source Title
VETERINARY SCIENCES
Year
2021
Volume
8
Issue
3
Page
-
Open Access
Green Published, gold
Publisher
MDPI
DOI
10.3390/vetsci8030039
Format
Abstract
We conducted a survey of canine microfilaraemia in 768 dogs in Chanthaburi, Samut Sakhon, and Narathiwat provinces of Thailand using a novel semi-automated, microfluidic device that is easy and rapid to perform. Microfilariae species were identified using High Resolution Melting real-time PCR (HRM real-time PCR). The prevalence of canine microfilaremia was 16.2% (45/278) in Chanthaburi and 5.5% (12/217) in Samut Sakhon. The prevalence of canine microfilaremia in Narathiwat was 22.7% (67/273). Brugia pahangi and Dirofilaria immitis were the predominant species of filariae found in the infected dogs from Chanthaburi and Narathiwat, respectively. The low prevalence of canine microfilaremia of Samut Sakhon may reflect the success of the Soi Dog foundation's efforts and the establishment of veterinary control programs. An effective disease control and prevention strategies is needed in Chanthaburi and Narathiwat to reduce the risks of zoonotic transmission of the parasites. An appropriate drug treatment should be given to infected dogs and prophylactic drugs are suggested to be given to dogs age <= 1-year-old to prevent filarial infection. The novel microfluidic device could be implemented for surveillance of filariae infection in other animals.
Keyword
Brugia malayi | Brugia pahangi | Dirofilaria immitis | HRM real time PCR | microfluidic
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Funding Sponsor
Faculty of Medicine at Siriraj Hospital, Mahidol University [R016233015]
License
CC BY
Rights
Authors
Publication Source
WOS