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Antimalarial alkaloids from a Bhutanese traditional medicinal plant Corydalis dubia
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Document Title
Antimalarial alkaloids from a Bhutanese traditional medicinal plant Corydalis dubia
Author
Wangchuk P, Keller PA, Pyne SG, Willis AC, Kamchonwongpaisan S
Name from Authors Collection
Affiliations
University of Wollongong; Australian National University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
JOURNAL OF ETHNOPHARMACOLOGY
ISSN
0378-8741
Year
2012
Volume
143
Issue
1
Page
310-313
Open Access
Green Submitted
Publisher
ELSEVIER IRELAND LTD
DOI
10.1016/j.jep.2012.06.037
Format
Abstract
Ethnopharmacological relevance: Corydalis dubia is used in Bhutanese traditional medicine as a febrifuge and for treating infections in the blood, liver and bile which correlate to the signs and symptoms of malarial and microbial infections. Aim of the study: To validate the ethnopharmacological uses of the plant and to discover potential new therapeutic drug leads. Materials and methods: C. dubia was collected from Bhutan and the alkaloids were obtained using acid base fractionation and separation by repeated column and preparative plate chromatography. The alkaloids were identified from analysis of their physiochemical and spectroscopic data and were tested for antiplasmodial, antimicrobial and cytotoxicity activities. Results: A systematic extraction and isolation protocol yielded one new natural product, dubiamine, and seven known isoquinoline alkaloids, scoulerine, cheilanthifoline, protopine, capnoidine, bicuculline, corydecumbine and hydrastine. Among the four alkaloids tested, scoulerine showed the best antiplasmodial activity with IC50 values of 5.4 mu M and 3.1 mu M against the antifolate sensitive and the multidrug resistant P. falciparum strains: TM4/8.2 and K1CB1, respectively. None of the alkaloids tested showed significant antimicrobial or cytotoxicity activities. Conclusions: The antiplasmodial test results, of the isolated alkaloid components, are commensurated with the ethnopharmacological uses of this plant. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Funding Sponsor
government of Australia
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Publication Source
WOS