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A novel dwarfing mutation in a green revolution gene from Brassica rapa
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Document Title
A novel dwarfing mutation in a green revolution gene from Brassica rapa
Author
Muangprom A, Thomas SG, Sun TP, Osborn TC
Name from Authors Collection
Affiliations
Duke University; University of Wisconsin System; University of Wisconsin Madison; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
PLANT PHYSIOLOGY
ISSN
0032-0889
Year
2005
Volume
137
Issue
3
Page
931-938
Open Access
Green Submitted, Green Published, Bronze
Publisher
AMER SOC PLANT BIOLOGISTS
DOI
10.1104/pp.104.057646
Format
Abstract
Mutations in the biosynthesis or signaling pathways of gibberellin (GA) can cause dwarfing phenotypes in plants, and the use of such mutations in plant breeding was a major factor in the success of the Green Revolution. DELLA proteins are GA signaling repressors whose functions are conserved in different plant species. Recent studies show that GA promotes stem growth by causing degradation of DELLA proteins via the ubiquitin-proteasome pathway. The most widely utilized dwarfing alleles in wheat (Triticum aestivum; e.g. Rht-B1b and Rht-D1b) encode GA-resistant forms of a DELLA protein that function as dominant and constitutively active repressors of stem growth. All of the previously identified dominant DELLA repressors from several plant species contain N-terminal mutations. Here we report on a novel dwarf mutant from Brassica rapa (Brrga1-d) that is caused by substitution of a conserved amino acid in the C-terminal domain of a DELLA protein. Brrga1-d, like N-terminal DELLA mutants, retains its repressor function and accumulates to high levels, even in the presence of GA. However, unlike wild-type and N-terminal DELLA mutants, Brrga1-d does not interact with a protein component required for degradation, suggesting that the mutated amino acid causes dwarfism by preventing an interaction needed for its degradation. This novel mutation confers nondeleterious dwarf phenotypes when transferred to Arabidopsis (Arabidopsis thaliana) and oilseed rape (Brassica napus), indicating its potential usefulness in other crop species.
Funding Sponsor
Biotechnology and Biological Sciences Research Council [P19317] Funding Source: Medline
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WOS