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Three-dimensional in vivo imaging by a handheld dual-axes confocal microscope
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Document Title
Three-dimensional in vivo imaging by a handheld dual-axes confocal microscope
Author
Ra HJ, Piyawattanametha W, Mandella MJ, Hsiung PL, Hardy J, Wang TD, Contag CH, Kino GS, Solgaard O
Name from Authors Collection
Affiliations
Stanford University; National Science & Technology Development Agency - Thailand; National Electronics & Computer Technology Center (NECTEC); National Nanotechnology Center (NANOTEC); Stanford University; Stanford University; University of Michigan System; University of Michigan
Type
Article
Source Title
OPTICS EXPRESS
ISSN
1094-4087
Year
2008
Volume
16
Issue
10
Page
7224-7232
Open Access
Green Accepted, gold
Publisher
OPTICAL SOC AMER
DOI
10.1364/OE.16.007224
Format
Abstract
We present a handheld dual-axes confocal microscope that is based on a two-dimensional microelectromechanical systems (MEMS) scanner. It performs reflectance and fluorescence imaging at 488 nm wavelength, with three-dimensional imaging capability. The fully packaged microscope has a diameter of 10 mm and acquires images at 4 Hz frame rate with a maximum field of view of 400 mu m x 260 mu m. The transverse and axial resolutions of the handheld probe are 1.7 mu m and 5.8 mu m, respectively. Capability to perform real time small animal imaging is demonstrated in vivo in transgenic mice. (C) 2008 Optical Society of America.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Funding Sponsor
NCI NIH HHS [U54 CA105296-04, R33 CA109988, R33 CA109988-04, U54 CA105296] Funding Source: Medline; NATIONAL CANCER INSTITUTE [U54CA105296, R33CA109988] Funding Source: NIH RePORTER
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Publication Source
WOS