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Application of raw starch degrading enzyme from Laceyella sacchari LP175 for development of bacterial cellulose fermentation using colored rice as substrate
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Document Title
Application of raw starch degrading enzyme from Laceyella sacchari LP175 for development of bacterial cellulose fermentation using colored rice as substrate
Author
Noree S, Tongdang C, Sujarit K, Chamdit S, Thongpool V, Trakarnpaiboon S, Khunnamwong P, Kitpreechavanich V, Lomthong T
Name from Authors Collection
Affiliations
Rajamangala University of Technology Thanyaburi; Ministry of Public Health - Thailand; Rajamangala University of Technology Thanyaburi; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Kasetsart University
Type
Article
Source Title
3 BIOTECH
ISSN
2190-572X
Year
2021
Volume
11
Issue
3
Page
-
Open Access
Green Published
Publisher
SPRINGER HEIDELBERG
DOI
10.1007/s13205-021-02673-3
Format
Abstract
Brown and black rice substrates were applied for sugar syrup production by the hydrolysis of raw starch degrading enzyme (RSDE) from Laceyella sacchari LP175 (300 U/mL) and commercial glucoamylase (GA, 2.0 U/mL) at 50 degrees C for 12 h using a simplex centroid mixture design. Results indicated that 300 g/L of substrates, consisting of 255 g/L Leum Pua glutinous rice and 45 g/L Black Jasmine rice, gave the highest sugar syrup production at 124.6 +/- 2.52 g/L with 2.00 +/- 0.05 mg GAE/mL of total phenolic content (TPC), equivalent to 0.42 +/- 0.01 g/g rice sample and 6.67 +/- 0.15 mg GAE/g rice sample, respectively. The obtained sugar syrup was used as the substrate for production of bacterial cellulose (Nata) by Komagataeibacter xylinus AGR 60 in a plastic tray at room temperature for 9 days. The fermentation medium containing 200 mL of rice syrup (25 g/L), 2.0 g of ammonium sulfate [(NH4)(2)SO4] and 0.4 mL glacial acetic acid yielded 1.1 +/- 0.08 cm thickness with 8.15 +/- 0.12 g of dry weight. The obtained bacterial cellulose from colored rice was characterized compared with bacterial cellulose from the conventional coconut juice by scanning electron microscope (SEM) and Fourier-transform infrared spectroscopy (FTIR) which demonstrated that the sugar syrup from colored rice could use as substrate for a novel bacterial cellulose as a healthy product in the future through microbial enzyme technological process.
Funding Sponsor
RMUTT Research Foundation Scholarship [DRF63D0606]
License
Copyright
Rights
King Abdulaziz City for Science and Technology
Publication Source
WOS