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Core genome sequence analysis to characterize Salmonella enterica serovar Rissen ST469 from a swine production chain
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Metadata
Document Title
Core genome sequence analysis to characterize Salmonella enterica serovar Rissen ST469 from a swine production chain
Author
Prasertsee T, Chuammitri P, Deeudom M, Chokesajjawatee N, Santiyanont P, Tadee P, Nuangmek A, Tadee P, Sheppard SK, Pascoe B, Patchanee P
Name from Authors Collection
Scopus Author ID
57189353906
Scopus Author ID
57189353906
Affiliations
Chiang Mai University; Chiang Mai University; Chiang Mai University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Chiang Mai University; Maejo University; University of Bath; Chiang Mai University
Type
Article
Source Title
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
ISSN
0168-1605
Year
2019
Volume
304
Page
68-74
Open Access
Green Submitted
Publisher
ELSEVIER SCIENCE BV
DOI
10.1016/j.ijfoodmicro.2019.05.022
Format
Abstract
Salmonella enterica subsp. enterica serotype Rissen is the predominant serotype found in Thai pork production and can be transmitted to humans through contamination of the food chain. This study was conducted to investigate the genetic relationships between serovar Rissen isolates from all levels of the pork production chain and evaluate the ability of the in silica antimicrobial resistance (AMR) genotypes to predict the phenotype of serovar Rissen. A total of 38 serovar Rissen isolates were tested against eight antibiotic agents by a disk diffusion method and the whole genomes of all isolates were sequenced to detect AMR genetic elements using the ResFinder database.A total of 86.84% of the isolates were resistant to tetracycline, followed by ampicillin (78.96%) and sulfonamide-trimethoprim (71.05%). Resistance to more than one antimicrobial agent was observed in 78.95% of the isolates, with the most common pattern showing resistance to ampicillin, chloramphenicol, streptomycin, sulfonamide-trimethoprim, and tetracycline. The results of genotypic AMR indicated that 89.47% of the isolates carried tet(A), 84.22% carried bla(TEM-1B), 78.95% carried sul3, and 78.95% carried dfrA12. The genotypic prediction of phenotypic resistance resulted in a mean sensitivity of 97.45% and specificity of 75.48%. Analysis by core genome multilocus sequence typing (cgMLST) demonstrated that the Salmonella isolates from various sources and different locations shared many of the same core genome loci. This implies that serovar Rissen has infected every stage of the pork production process and that contamination can occur in every part of the production chain.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Funding Sponsor
Royal Golden Jubilee (RGJ); Programme, Thailand Research Fund (TRF); Chiang Mai University; National Science and Technology Development Agency (NSTDA) [P-11-00729, P-10-10409]
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Rights
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Publication Source
WOS