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Taxonomic and Metabolite Diversities of Moss-Associated Actinobacteria from Thailand
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Metadata
Document Title
Taxonomic and Metabolite Diversities of Moss-Associated Actinobacteria from Thailand
Author
Insuk C, Pongpamorn P, Forsythe A, Matsumoto A, Omura S, Pathom-aree W, Cheeptham N, Xu JP
Name from Authors Collection
Affiliations
McMaster University; National Science & Technology Development Agency - Thailand; Kitasato University; Chiang Mai University
Type
Article
Source Title
METABOLITES
Year
2022
Volume
12
Issue
1
Open Access
Green Published, gold
Publisher
MDPI
DOI
10.3390/metabo12010022
Format
Abstract
Actinobacteria are a group of ecologically important bacteria capable of producing diverse bioactive compounds. However, much remains unknown about the taxonomic and metabolic diversities of actinobacteria from many geographic regions and ecological niches. In this study, we report the isolation of actinobacteria from moss and moss-associated rhizosphere soils in Thailand. Among the 89 isolates analyzed for their bioactivities, 86 strains produced indole-3-acetic acid (IAA, ranging from 0.04 to 59.12 mg/L); 42 strains produced hydroxamate type of siderophore; 35 strains produced catecholate type of siderophore; 21 strains solubilized tricalcium phosphate; and many strains exhibited antagonistic activities against one to several of the seven selected plant, animal, and human pathogens. Overall, actinobacteria from the rhizosphere soil of mosses showed greater abilities to produce IAA and siderophores and to solubilize tricalcium phosphate than those from mosses. Among these 89 isolates, 37 were analyzed for their 16S rRNA gene sequences, which revealed their diverse phylogenetic distributions among seven genera, Streptomyces, Micromonospora, Nocardia, Actinoplanes, Saccharothrix, Streptosporangium, and Cryptosporangium. Furthermore, gas chromatography-mass spectrometry analyses of ethyl acetate crude extracts of three selected isolates with inhibitory effects against a methicillin-resistant Staphylococcus aureus strain revealed diverse metabolites with known antimicrobial activities. Together, our results demonstrate that actinobacteria from mosses in Thailand are taxonomically diverse and capable of producing a range of metabolites with plant-growth-promoting and microbial pathogen-inhibiting potentials.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC-BY
Rights
Authors
Publication Source
WOS