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Enhanced antibacterial effect of a novel Friunavirus phage vWU2001 in combination with colistin against carbapenem-resistant Acinetobacter baumannii
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Metadata
Document Title
Enhanced antibacterial effect of a novel Friunavirus phage vWU2001 in combination with colistin against carbapenem-resistant Acinetobacter baumannii
Author
Wintachai P, Phaonakrop N, Roytrakul S, Naknaen A, Pomwised R, Voravuthikunchai SP, Surachat K, Smith DR
Name from Authors Collection
Affiliations
Walailak University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Prince of Songkla University; Prince of Songkla University; Prince of Songkla University; Prince of Songkla University; Mahidol University
Type
Article
Source Title
SCIENTIFIC REPORTS
Year
2022
Volume
12
Issue
1
Open Access
Green Published, gold
Publisher
NATURE PORTFOLIO
DOI
10.1038/s41598-022-06582-0
Format
Abstract
The emergence of carbapenem-resistant Acinetobacter baumannii (CRAB) has been increasingly reported, leading to greater challenges in treating infections. With the development of phage therapy and phage-antibiotic combinations, it is promising to improve the treatment of bacterial infections. In the present study, a novel vB_AbaP_WU2001 (vWU2001) phage-specific CRAB with a genome of 40,792 bp was isolated. Genomic analysis disclosed that it belongs to the Autographiviridae family of the order Caudovirales. Phage vWU2001 had a broad host range with a high adsorption rate, short latent period, large burst size and good stability. The phage could reduce preformed biofilms and inhibit biofilm formation. The combination of phage vWU2001 and colistin had significantly higher bacterial growth inhibition activity than that of phage, or colistin alone. The efficacy of the combined treatment was also evaluated in Galleria mellonella. Evaluation of its therapeutic potential showed that the combination of phage and colistin resulted in a significantly greater increase in G. mellonella survival and in bacterial clearance, as compared with that of phage or colistin alone, indicating that the combination was synergistic against CRAB. The results demonstrated that phage vWU2001 has the potential to be developed as an antibacterial agent.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Walailak University, Thailand [WU63216, CGS-P2P-2564-068]; Thai Government, Thailand [SCI560059S]; National Research Council of Thailand, Thailand [N41A640071]; Faculty of Science, Prince of Songkla University [SCI6404013S]
License
CC-BY
Rights
Authors
Publication Source
WOS