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Genome-scale metabolic modeling of Mucor circinelloides and comparative analysis with other oleaginous species
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Document Title
Genome-scale metabolic modeling of Mucor circinelloides and comparative analysis with other oleaginous species
Author
Vongsangnak W, Klanchui A, Tawornsamretkit I, Tatiyaborwornchai W, Laoteng K, Meechai A
Name from Authors Collection
Affiliations
Kasetsart University; Soochow University - China; King Mongkuts University of Technology Thonburi; King Mongkuts University of Technology Thonburi; King Mongkuts University of Technology Thonburi; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Kasetsart University
Type
Article
Source Title
GENE
Year
2016
Volume
583
Issue
2
Page
121-129
Open Access
Bronze
Publisher
ELSEVIER
DOI
10.1016/j.gene.2016.02.028
Format
Abstract
We present a novel genome-scale metabolic model iWV1213 of Mucor circinelloides, which is an oleaginous fungus for industrial applications. The model contains 1213 genes, 1413 metabolites and 1326 metabolic reactions across different compartments. We demonstrate that iWV1213 is able to accurately predict the growth rates of M. circinelloides on various nutrient sources and culture conditions using Flux Balance Analysis and Phenotypic Phase Plane analysis. Comparative analysis of three oleaginous genome-scale models, including M. circinelloides (iWV1213), Mortierella alpina (iCY1106) and Yarrowia lipolytica (iYL619_PCP) revealed that iWV1213 possesses a higher number of genes involved in carbohydrate, amino acid, and lipid metabolisms that might contribute to its versatility in nutrient utilization. Moreover, the identification of unique and common active reactions among the Zygomycetes oleaginous models using Flux Variability Analysis unveiled a set of gene/enzyme candidates as metabolic engineering targets for cellular improvement. Thus, iWV1213 offers a powerful metabolic engineering tool for multi-level omics analysis, enabling strain optimization as a cell factory platform of lipid-based production. (C) 2016 Elsevier B.V. All rights reserved.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Natural Science Foundation of China (NSFC) [31200989]; Thailand Research Fund [TRG5880245]; Preproposal Research Fund [PRF4/2558]
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Publication Source
WOS