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Identification of Acetobacter strains isolated in Thailand based on the phenotypic, chemotaxonomic, and molecular characterizations
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Document Title
Identification of Acetobacter strains isolated in Thailand based on the phenotypic, chemotaxonomic, and molecular characterizations
Author
Kommanee J, Tanasupawat S, Yukphan P, Thongchul N, Moonmangmee D, Yamada Y
Name from Authors Collection
Affiliations
Chulalongkorn University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Chulalongkorn University; King Mongkuts University of Technology Thonburi; Shizuoka University
Type
Article
Source Title
SCIENCEASIA
Year
2012
Volume
38
Issue
1
Page
44-53
Open Access
Bronze
Publisher
SCIENCE SOCIETY THAILAND
DOI
10.2306/scienceasia1513-1874.2012.38.044
Format
Abstract
The restriction analysis of 16S-23S rRNA gene internal transcribed spacer regions (ITS) using TaqI, AluI, HpaII, and AvaII revealed that forty-seven bacterial isolates found in fruits and flowers collected in Thailand belong to the genus Acetobacter. They were identified as follows: Group 1 containing 11 isolates was identified as A. pasteurianus, Group 2 containing nine isolates was identified as A. orientalis, Group 3 containing eight isolates was identified as A. lovaniensis, Group 4 including eight isolates was identified as A. indonesiensis, Group 5 containing three isolates was identified as A. tropicalis, Group 6 containing five isolates was identified as A. ghanensis, and Group 7 containing four isolates was identified as A. orleanensis. The differentiation of Acetobacter species by the 16S-23S rRNA gene ITS restriction analysis was discussed. The phenotypic, chemotaxonomic, and molecular characteristics including 16S rRNA gene sequences supported the above-mentioned identification. All Thai isolates were screened for their alcohol dehydrogenase activity and exhibited an activity of 2.05-7.52 units/mg protein at 30 degrees C. Isolate PHD-23 produced the highest quantity of 1.50% acetic acid (v/v) from 4.0% ethanol (v/v).
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Faculty of Pharmaceutical Sciences, Chulalongkorn University, Thailand; Graduate Institute of Science and Technology, TGIST, National Science and Technology Development Agency, Thailand
Publication Source
WOS