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A wild rice-derived peptide R14 ameliorates monosodium urate crystals-induced IL-1? secretion through inhibition of NF-?B signaling and NLRP3 inflammasome activation
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Document Title
A wild rice-derived peptide R14 ameliorates monosodium urate crystals-induced IL-1? secretion through inhibition of NF-?B signaling and NLRP3 inflammasome activation
Author
Charoenwutthikun S., Chanjitwiriya K., Roytrakul S., Kunthalert D.
Affiliations
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Thailand Science Park, Pathumthani, Thailand; Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand
Type
Article
Source Title
PeerJ
ISSN
21678359
Year
2023
Volume
11
Open Access
All Open Access, Gold, Green
Publisher
PeerJ Inc.
DOI
10.7717/PEERJ.15295
Format
Abstract
Gout is an inflammatory arthritis initiated by the deposition of monosodium urate crystals (MSU) around the joints and surrounding tissues. MSU crystals activate the nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 (NLRP3) inflammasome to the release of interleukin-1? (IL-1?). Gout can have a substantial impact on patient's quality of life, and currently available medicines are unable to meet all the clinical needs. This study explored anti-gout potentials of the Rice14 (R14) peptide, a peptide derived from leaves of wild rice Oryza minuta. The effects of R14 peptide on IL-1? secretion in THP-1 macrophages with MSU crystals-induced inflammation were examined. Our results clearly showed that the R14 peptide significantly inhibited the secretion of IL-1? in MSU crystals-induced macrophages, and the effects were dose-related. For safety testing, the R14 peptide did not show both cytotoxicity and hemolytic activity. In addition, the R14 peptide strongly suppressed the phospho-I?B-? and nuclear factor kappa-B (NF-?B) p65 proteins in NF-?B signaling pathway, reduced the NLRP3 expression and inhibited the MSU crystals-mediated cleavage of caspase-1 as well as mature IL-1?. The R14 peptide also reduced MSU-triggered intracellular ROS levels in macrophages. Taken together, these results indicated that R14 peptide inhibited MSU crystals-induced IL-1? production through NF-?B and NLRP3 inflammasome activation. Our findings demonstrated that R14 peptide, the newly recognized peptide from wild rice, possessed potent regulatory activity against IL-1? production in MSU crystals-induced inflammation, and we therefore propose that the R14 peptide is a promising molecule with potential clinical application in the treatment of MSU crystals-induced inflammation. Copyright ? 2023 Charoenwutthikun et al.
Keyword
Gout | Inflammasome | Interleukin-1? | Monosodium urate | NF-?B
License
CC BY
Rights
Authors
Publication Source
WOS