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Unraveling the photophysical characteristics and biological applications of vinyl sulfones as viscosity sensors
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Document Title
Unraveling the photophysical characteristics and biological applications of vinyl sulfones as viscosity sensors
Author
Khaikate O., Pewklang T., Khrootkaew T., Chansaenpak K., Muangsopa P., Kuhakarn C., Kamkaew A.
Affiliations
School of Chemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand; National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathum Thani, 12120, Thailand; Department of Chemistry and Center of Excellence for Innovation in Chemistry (PERCH-CIC), Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, 10400, Thailand
Type
Article
Source Title
RSC Advances
ISSN
20462069
Year
2023
Volume
13
Issue
24
Page
16671-16677
Open Access
All Open Access, Gold, Green
Publisher
Royal Society of Chemistry
DOI
10.1039/d3ra02354k
Format
Abstract
For the first time, a series of vinyl sulfone-NH2-based push-pull fluorophores (4a-4d) were introduced for their potential use in biological applications. The fluorophores 4a-4d were readily synthesized upon reduction of the corresponding vinyl sulfones-NO2 (3a-3d), which were prepared by sulfonylation of nitrostyrene. Both types of probes can be prepared in high yields through a few steps with minimal cost. In diverse solvents, probes 4a-4d exhibited fluorescence with strong emission peaking around 403-490 nm. Additionally, the fluorescence intensity of probe 4d rose approximately 85-fold with increasing viscosity. The probes 4a-4d demonstrated good stability and photostability in a broad pH range. Moreover, probes 4a-4d showed significantly improved biocompatibility compared to those derived from 3a-3d. For cell imaging applications, the developed probes 4a-4d exhibited much stronger blue fluorescence in cancer cells (HepG2) compared to 3a-3d. In addition, probes 4a-4d exhibited low cytotoxicity within 24 h toward both cancer and normal cells (HEK-293). Interestingly, probe 4d showed great sensitivity to viscosity in cancer cells. As a result, readily prepared vinyl sulfone-NH2-based push-pull fluorophores (4a-4d) offer a promising strategy for further development as cancer cell staining agents. ? 2023 The Royal Society of Chemistry.
License
CC BY-NC
Rights
Authors
Publication Source
WOS