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Proteomic analysis of pulmonary arteries and lung tissues from dogs affected with pulmonary hypertension secondary to degenerative mitral valve disease
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Document Title
Proteomic analysis of pulmonary arteries and lung tissues from dogs affected with pulmonary hypertension secondary to degenerative mitral valve disease
Author
Sakarin S. Rungsipipat A. Roytrakul S. Jaresitthikunchai J. Phaonakrop N. Charoenlappanit S. Thaisakun S. Surachetpong S.D.
Affiliations
Department of Veterinary Medicine Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand; Center of Excellence for Companion Animal Cancer Department of Pathology Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand; Functional Proteomics Technology Laboratory National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency Pathum Thani Thailand
Type
Article
Source Title
PLoS ONE
ISSN
19326203
Year
2024
Volume
19
Issue
1-�.�.
Open Access
All Open Access Gold Green
Publisher
Public Library of Science
DOI
10.1371/journal.pone.0296068
Abstract
In dogs with degenerative mitral valve disease (DMVD) pulmonary hypertension (PH) is a common complication characterized by abnormally elevated pulmonary arterial pressure (PAP). Pulmonary arterial remodeling is the histopathological changes of pulmonary artery that has been recognized in PH. The underlying mechanisms that cause this arterial remodeling are poorly understood. This study aimed to perform shotgun proteomics to investigate changes in protein expression in pulmonary arteries and lung tissues of DMVD dogs with PH compared to normal control dogs and DMVD dogs without PH. Tissue samples were collected from the carcasses of 22 small-sized breed dogs and divided into three groups: control (n = 7) DMVD (n = 7) and DMVD+PH groups (n = 8). Differentially expressed proteins were identified and top three upregulated and downregulated proteins in the pulmonary arteries of DMVD dogs with PH including SIK family kinase 3 (SIK3) Collagen type I alpha 1 chain (COL1A1) Transforming growth factor alpha (TGF-?) Apoptosis associated tyrosine kinase (AATYK) Hepatocyte growth factor activator (HGFA) and Tyrosine-protein phosphatase non-receptor type 13 (PTPN13) were chosen. Results showed that some of the identified proteins may play a role in the pathogenesis of pulmonary arterial remodeling. This study concluded shotgun proteomics has potential as a tool for exploring candidate proteins associated with the pathogenesis of PH secondary to DMVD in dogs. ? 2024 Sakarin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use distribution and reproduction in any medium provided the original author and source are credited.
License
CC BY
Rights
Authors
Publication Source
WOS