Comparative Serum Proteome Profiling of Canine Benign Prostatic Hyperplasia before and after Castration

Abstract

BPH is the most prevalent prostatic condition in aging dogs. Nevertheless clinical diagnosis and management remain inconsistent. This study employed in-solution digestion coupled with nano-liquid chromatography tandem mass spectrometry to assess serum proteome profiling of dogs with BPH and those dogs after castration. Male dogs were divided into two groups; control and BPH groups. In the BPH group each dog was evaluated at two time points: Day 0 (BF subgroup) and Day 30 after castration (AT subgroup). In the BF subgroup three proteins were significantly upregulated and associated with dihydrotestosterone: solute carrier family 5 member 5 tyrosine-protein kinase and FRAT regulator of WNT signaling pathway 1. Additionally the overexpression of polymeric immunoglobulin receptors in the BF subgroup hints at its potential as a novel protein linked to the BPH development process. Conversely alpha-1-B glycoprotein (A1BG) displayed significant downregulation in the BF subgroup suggesting A1BG抯 potential as a predictive protein for canine BPH. Finasteride was associated with increased proteins in the AT subgroup including apolipoprotein C-I apolipoprotein E apolipoprotein A-II TAO kinase 1 DnaJ homolog subfamily C member 16 PH domain and leucine-rich repeat protein phosphatase 1 neuregulin 1 and pseudopodium enriched atypical kinase 1. In conclusion this pilot study highlighted alterations in various serum proteins in canine BPH reflecting different pathological changes occurring in this condition. These proteins could be a source of potential non-invasive biomarkers for diagnosing this disease. ? 2023 by the authors.