-
Resveratrol Loaded Liposomes Disrupt Cancer Associated Fibroblast Communications within the Tumor Microenvironment to Inhibit Colorectal Cancer Aggressiveness
- Back
Document Title
Resveratrol Loaded Liposomes Disrupt Cancer Associated Fibroblast Communications within the Tumor Microenvironment to Inhibit Colorectal Cancer Aggressiveness
Author
Dana P. Thumrongsiri N. Tanyapanyachon P. Chonniyom W. Punnakitikashem P. Saengkrit N.
Affiliations
National Nanotechnology Center (NANOTEC) National Science and Technology Development Agency (NSTDA) Pathum Thani12120 Thailand; Department of Biochemistry Faculty of Medicine Siriraj Hospital Mahidol University 2 Wanglang Road Bangkoknoi Bangkok 10700 Thailand; Research Network NANOTEC-Mahidol University in Theranostic Nanomedicine Faculty of Medicine Siriraj Hospital Mahidol University 2 Wanglang Road Bangkoknoi Bangkok 10700 Thailand
Type
Article
Source Title
Nanomaterials
ISSN
20794991
Year
2023
Volume
13
Issue
1
Open Access
All Open Access Gold Green
Publisher
MDPI
DOI
10.3390/nano13010107
Abstract
Colorectal cancer (CRC) is a cancer-associated fibroblast CAF-rich tumor. CAF promotes cancer cell proliferation metastasis drug resistance via secretes soluble factors and extracellular matrices which leads to dense stroma a major barrier for drug delivery. Resveratrol (RES) is a polyphenolic compound has several pharmacologic functions including anti-inflammation and anticancer effects. Considering tumor microenvironment of CRC resveratrol-loaded liposome (L-RES) was synthesized and employed to inhibit CAF functions. The L-RES was synthesized by thin-film hydration method. The cytotoxicity of L-RES was evaluated using MTT assay. Effect of L-RES treated CAF on tumor spheroid growth was performed. Cell invasion was determined using spheroid invasion assay. The effect of L-RES on 5-fluorouracil (5-FU) sensitivity of CRC cells was determined in co-cultured tumor spheroids. Subtoxic dose of L-RES was selected to study possible inhibiting CAF functions. Decreased CAF markers ?-SMA and IL-6 levels were observed in L-RES treated activated fibroblast. Interestingly the activated fibroblast promoted invasive ability and drug resistance of CRC cells in co-culture condition of both 2D and 3D cultures and was attenuated by L-RES treatment in the activated fibroblast. Therefore L-RES provides a promising drug delivery strategy for CRC treatment by disrupting the crosstalk between CRC cells and CAF. ? 2022 by the authors.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
Scopus