• Optimizing protein delivery rate from silk fibroin hydrogel using silk fibroin-mimetic peptides conjugation

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Document Title

Optimizing protein delivery rate from silk fibroin hydrogel using silk fibroin-mimetic peptides conjugation

Author

Promsuk J. Manissorn J. Laomeephol C. Luckanagul J.A. Methachittipan A. Tonsomboon K. Jenjob R. Yang S.-G. Thongnuek P. Wangkanont K.

Affiliations

Department of Biochemistry Center of Excellence for Molecular Biology and Genomics of Shrimp Faculty of Science Chulalongkorn University Bangkok 10330 Thailand; Department of Biochemistry Center of Excellence in Molecular Crop Faculty of Science Chulalongkorn University Bangkok 10330 Thailand; Biomedical Materials and Devices for Revolutionary Integrative Systems Engineering Research Unit (BMD-RISE) Faculty of Engineering Chulalongkorn University Bangkok 10330 Thailand; Department of Pharmaceutics and Industrial Pharmacy Faculty of Pharmaceutical Sciences Chulalongkorn University Bangkok 10330 Thailand; Nano Engineering Program International School of Engineering Faculty of Engineering Chulalongkorn University Bangkok 10330 Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Pathum Thani Khlong Luang 12120 Thailand; Department of Biomedical Science BK21 FOUR Program in Biomedical Science and Engineering Inha University College of Medicine Incheon 22332 South Korea; Biomedical Engineering Program Faculty of Engineering Chulalongkorn University Bangkok 10330 Thailand; Biomedical Engineering Research Center Faculty of Engineering Chulalongkorn University Bangkok 10330 Thailand

Type

Article

Source Title

Scientific Reports

ISSN

20452322

Year

2024

Volume

14

Issue

1

Open Access

All Open Access Gold

Publisher

Nature Research

DOI

10.1038/s41598-024-53689-7

Abstract

Controlled release of proteins such as growth factors from biocompatible silk fibroin (SF) hydrogel is valuable for its use in tissue engineering drug delivery and other biological systems. To achieve this we introduced silk fibroin-mimetic peptides (SFMPs) with the repeating unit (GAGAGS)n. Using green fluorescent protein (GFP) as a model protein our results showed that SFMPs did not affect the GFP function when conjugated to it. The SFMP-GFP conjugates incorporated into SF hydrogel did not change the gelation time and allowed for controlled release of the GFP. By varying the length of SFMPs we were able to modulate the release rate with longer SFMPs resulting in a slower release both in water at room temperature and PBS at 37�C. Furthermore the SF hydrogel with the SFMPs showed greater strength and stiffness. The increased ?-sheet fraction of the SF hydrogel as revealed by FTIR analysis explained the gel properties and protein release behavior. Our results suggest that the SFMPs effectively control protein release from SF hydrogel with the potential to enhance its mechanical stability. The ability to modulate release rates by varying the SFMP length will benefit personalized and controlled protein delivery in various systems. ? The Author(s) 2024.

Industrial Classification

N/A

Knowledge Taxonomy Level 1

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Knowledge Taxonomy Level 2

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Knowledge Taxonomy Level 3

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License

CC BY

Rights

Authors

Publication Source

Scopus

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