-
Cardiac endothelial ischemia/reperfusion injury-derived protein damage-associated molecular patterns disrupt the integrity of the endothelial barrier
- Back
Document Title
Cardiac endothelial ischemia/reperfusion injury-derived protein damage-associated molecular patterns disrupt the integrity of the endothelial barrier
Author
Kumphune S. Seenak P. Paiyabhrom N. Songjang W. Pankhong P. Jumroon N. Thaisakun S. Phaonakrop N. Roytrakul S. Malakul W. Jiraviriyakul A. Nernpermpisooth N.
Affiliations
Biomedical Engineering and Innovation Research Centre Chiang Mai University Muang Chiang Mai 50200 Thailand; Biomedical Engineering Institute Chiang Mai University Muang Chiang Mai 50200 Thailand; Integrative Biomedical Research Unit (IBRU) Faculty of Allied Health Sciences Naresuan University Phitsanulok 65000 Thailand; Department of Cardio-Thoracic Technology Faculty of Allied Health Sciences Naresuan University Phitsanulok 65000 Thailand; Department of Medical Technology Faculty of Allied Health Sciences Naresuan University Phitsanulok 65000 Thailand; National Centre for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency Pathum Thani12120 Thailand; Department of Physiology Faculty of Medical Science Naresuan University Phitsanulok 65000 Thailand
Type
Article
Source Title
Heliyon
ISSN
24058440
Year
2024
Volume
10
Issue
2
Open Access
All Open Access Gold
Publisher
Elsevier Ltd
DOI
10.1016/j.heliyon.2024.e24600
Abstract
Human cardiac microvascular endothelial cells (HCMECs) are sensitive to ischemia and vulnerable to damage during reperfusion. The release of damage-associated molecular patterns (DAMPs) during reperfusion induces additional tissue damage. The current study aimed to identify early protein DAMPs in human cardiac microvascular endothelial cells subjected to ischemia-reperfusion injury (IRI) using a proteomic approach and their effect on endothelial cell injury. HCMECs were subjected to 60 min of simulated ischemia and 6 h of reperfusion which can cause lethal damage. DAMPs in the culture media were subjected to liquid chromatography-tandem mass spectrometry proteomic analysis. The cells were treated with endothelial IRI-derived DAMP medium for 24 h. Endothelial injury was assessed by measuring lactate dehydrogenase activity morphological features and the expression of endothelial cadherin nitric oxide synthase (eNOS) and caveolin-1. The top two upregulated proteins DNAJ homolog subfamily B member 11 and pyrroline-5-carboxylate reductase 2 are promising and sensitive predictors of cardiac microvascular endothelial damage. HCMECs expose to endothelial IRI-derived DAMP the lactate dehydrogenase activity was significantly increased compared with the control group (10.15 ? 1.03 vs 17.67 ? 1.19 respectively). Following treatment with endothelial IRI-derived DAMPs actin-filament dysregulation and downregulation of vascular endothelial cadherin caveolin-1 and eNOS expressions were observed along with cell death. In conclusion the early protein DAMPs released during cardiac microvascular endothelial IRI could serve as novel candidate biomarkers for acute myocardial IRI. Distinct features of impaired plasma membrane integrity can help identify therapeutic targets to mitigate the detrimental consequences mediated of endothelial IRI-derived DAMPs. ? 2024 The Authors
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY-NC-ND
Rights
Authors
Publication Source
Scopus