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Non-targeted proteomic analysis of Asian elephant (Elephas maximus) seminal plasma using an in-solution digestion technique and liquid chromatography tandem-mass spectrometry
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Document Title
Non-targeted proteomic analysis of Asian elephant (Elephas maximus) seminal plasma using an in-solution digestion technique and liquid chromatography tandem-mass spectrometry
Author
Wattananit P. Yingchutrakul Y. Kornkaewrat K. Mahasawangkul S. Roytrakul S. Pinyopummin A.
Affiliations
Faculty of Veterinary Science Mahidol University Nakhon Pathom Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency Pathum Thani Thailand; Faculty of Veterinary Medicine Kasetsart University Nakhon Pathom Thailand; The National Elephant Institute The Forest Industry Organization Lampang Thailand
Type
Article
Source Title
Frontiers in Veterinary Science
ISSN
22971769
Year
2023
Volume
10
Open Access
All Open Access Gold Green
Publisher
Frontiers Media SA
DOI
10.3389/fvets.2023.1174078
Abstract
Seminal plasma proteins have recently been reported to play a significant role as valuable materials for understanding male reproductive biology identifying causes of fertility problems and developing reproductive biomarkers. Proteomic analysis of seminal plasma holds promise in advancing the understanding of male Asian elephant reproductive biology. This study aims to explore seminal plasma proteins of Asian elephants and their probable functions to provide fundamental information about male reproduction in this species. The protein solution from pooled seminal plasma from 10 bulls (a total of 33 ejaculates) was digested into peptides and identified using LC-MS/MS. Out of 986 proteins 597 were mapped and matched with 58 species in UniProt databases including Elephas maximus. These mapped proteins were mostly involved in binding function catalytic activity cellular process and metabolic process. Only 29 mapped proteins were recognized to be related in reproductive process mainly associated in spermatogenesis and sperm capacitation. Additionally several seminal plasma proteins related to fertility or semen quality in other mammals were also found in Asian elephant semen such as keratin type I aldose reductase thrombospondon-1 fibronectin 1 platelet-activating factor acetyl hydrolase mannosidase and semenogelin-2. This discovery clearly reveals the beneficial protein profile in seminal plasma of the Asian elephant and serves as a crucial step in investigating infertility and poor semen quality in this valuable species. Copyright ? 2023 Wattananit Yingchutrakul Kornkaewrat Mahasawangkul Roytrakul and Pinyopummin.
Keyword
Asian elephant | male reproduction | Protein | proteomic analysis | seminal plasma
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
Scopus