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Functional and genomic characterization of a novel probiotic Lactobacillus johnsonii KD1 against shrimp WSSV infection
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Document Title
Functional and genomic characterization of a novel probiotic Lactobacillus johnsonii KD1 against shrimp WSSV infection
Author
Dekham K. Jones S.M. Jitrakorn S. Charoonnart P. Thadtapong N. Intuy R. Dubbs P. Siripattanapipong S. Saksmerprome V. Chaturongakul S.
Affiliations
Department of Microbiology Faculty of Science Mahidol University Bangkok 10400 Thailand; School of Biosciences Division of Natural Sciences University of Kent Canterbury CT2 7NZ United Kingdom; National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Pathum Thani 12120 Thailand; Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp) Faculty of Science Mahidol University Bangkok 10400 Thailand; Graduate Program in Biomedical Sciences Faculty of Allied Health Sciences Thammasat University Pathum Thani 12120 Thailand; Molecular Medical Biosciences Cluster Institute of Molecular Biosciences Mahidol University Nakhon Pathom 73170 Thailand
Type
Article
Source Title
Scientific Reports
ISSN
20452322
Year
2023
Volume
13
Issue
1
Open Access
All Open Access Gold
Publisher
Nature Research
DOI
10.1038/s41598-023-47897-w
Abstract
White Spot syndrome virus (WSSV) causes rapid shrimp mortality and production loss worldwide. This study demonstrates potential use of Lactobacillus johnsonii KD1 as an anti-WSSV agent for post larva shrimp cultivation and explores some potential mechanisms behind the anti-WSSV properties. Treatment of Penaeus vannamei shrimps with L. johnsonii KD1 prior to oral challenge with WSSV-infected tissues showed a significantly reduced mortality. In addition WSSV copy numbers were not detected and shrimp immune genes were upregulated. Genomic analysis of L. johnsonii KD1 based on Illumina and Nanopore platforms revealed a 1.87燤b chromosome and one 15.4燢b plasmid. Only one antimicrobial resistance gene (ermB) in the chromosome was identified. Phylogenetic analysis comparing L. johnsonii KD1 to other L. johnsonii isolates revealed that L. johnsonii KD1 is closely related to L. johnsonii GHZ10a isolated from wild pigs. Interestingly L. johnsonii KD1 contains isolate-specific genes such as genes involved in a type I restriction-modification system and CAZymes belonging to the GT8 family. Furthermore genes coding for probiotic survival and potential antimicrobial/anti-viral metabolites such as a homolog of the bacteriocin helveticin-J were found. Protein杙rotein docking modelling suggests the helveticin-J homolog may be able to block VP28朠mRab7 interactions and interrupt WSSV infection. ? 2023 The Author(s).
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
WOS